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Titolo:
Determination of amino acids in human blood serum by reversed-phase high-performance liquid chromatography in the isocratic elution mode
Autore:
Krasnova, IN; Kartsova, LA; Cherkas, YV;
Indirizzi:
Russian Acad Sci, Inst Human Brain, St Petersburg 197376, Russia Russian Acad Sci St Petersburg Russia 197376 t Petersburg 197376, Russia St Petersburg State Univ, Dept Chem, St Petersburg 198904, Russia St Petersburg State Univ St Petersburg Russia 198904 burg 198904, Russia
Titolo Testata:
JOURNAL OF ANALYTICAL CHEMISTRY
fascicolo: 1, volume: 55, anno: 2000,
pagine: 58 - 65
SICI:
1061-9348(200001)55:1<58:DOAAIH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; PRECOLUMN DERIVATIZATION; SULFITE DERIVATIZATION; DERIVATIVES; PHTHALDIALDEHYDE; SEPARATION; SAMPLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Krasnova, IN Russian Acad Sci, Inst Human Brain, Ul Akad Pavlova 9, St Petersburg 197376, Russia Russian Acad Sci Ul Akad Pavlova 9 St Petersburg Russia 197376
Citazione:
I.N. Krasnova et al., "Determination of amino acids in human blood serum by reversed-phase high-performance liquid chromatography in the isocratic elution mode", J ANALYT CH, 55(1), 2000, pp. 58-65

Abstract

A procedure is developed for the determination of 15 amino acids in human blood serum using ortho-phthalic aldehyde in combination with 2-mercaptoethanol or sodium sulfite as the reagent for the precalumn synthesis of derivatives with their subsequent separation by reversed-phase high-performance liquid chromatography in the isocratic elution mode using electrochemical detection. Conditions of the quantitative conversion of amino acids to corresponding derivatives were determined; ortho-phthalic/2-mercaptoethanol and ortho-phthalic/sulfite derivatives of amino acids are stable during the whole cycle of analysis. The total time of separation is 80 min. The detection limits are 0.5-5 pmol (at the signal-to-noise ratio equal to 2). The procedure is used for the determination of glutamic acid, asparagine, serine, glutamine, histidine, taurine, alanine, arginine, methionine, isoleucine, ornithine, leucine, phenylalanine, lysine, and tryptophane in blood serum of healthy donors and of sick with alcoholism before: and after treatment.

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Documento generato il 04/04/20 alle ore 02:01:13