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Titolo:
Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy
Autore:
Kazemi-Shirazi, L; Pauli, G; Purohit, A; Spitzauer, S; Froschl, R; Hoffmann-Sommergruber, K; Breiteneder, H; Scheiner, O; Kraft, D; Valenta, R;
Indirizzi:
Univ Vienna, Inst Gen & Expt Pathol, Vienna, Austria Univ Vienna Vienna Austria nna, Inst Gen & Expt Pathol, Vienna, Austria Univ Vienna, Dept Internal Med 4, Div Gastroenterol & Hepatol, Vienna, Austria Univ Vienna Vienna Austria Div Gastroenterol & Hepatol, Vienna, Austria Univ Vienna, Clin Inst Med & Chem Lab Diagnost, AKH, Vienna, Austria Univ Vienna Vienna Austria ed & Chem Lab Diagnost, AKH, Vienna, Austria Univ Strasbourg, Hosp Civil, Serv Pneumol, Strasbourg, France Univ Strasbourg Strasbourg France vil, Serv Pneumol, Strasbourg, France Univ Strasbourg, Hosp Civil, INSERM, U425, Strasbourg, France Univ Strasbourg Strasbourg France vil, INSERM, U425, Strasbourg, France
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 1, volume: 105, anno: 2000,
parte:, 1
pagine: 116 - 125
SICI:
0091-6749(200001)105:1<116:QIIEWP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CROSS-REACTIVE ALLERGEN; BIRCH POLLEN; MAJOR ALLERGEN; IMMUNOLOGICAL RELATIONSHIP; VEGETABLE FOODS; TREE POLLEN; IDENTIFICATION; ANTIBODIES; PROFILIN; BET-V-1;
Keywords:
food allergy; pollen allergy; cross-reactivity; recombinant allergens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Valenta, R Univ Vienna, Vienna Gen Hosp, Inst Gen & Expt Pathol, Med Sch,Mol Immunopathol Grp, Waehringer Guertel 18-20, A-1090 Vienna, Austria Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090 ria
Citazione:
L. Kazemi-Shirazi et al., "Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy", J ALLERG CL, 105(1), 2000, pp. 116-125

Abstract

Background: Type I allergic symptoms in the oropharyngeal mucosa upon contact with plant-derived food in patients with pollen allergies have been termed oral allergy syndrome (OAS). IgE cross-reactivity between pollen and food allergens represents the molecular basis for this phenomenon. The sensitizing allergen source (pollen or plant Toed) in OAS is a controversial issue. Objective:We sought to determine the primary sensitizing molecules in patients with GAS. Methods: We used recombinant birch pollen (rBet v 1 and rBet v 2) and plant food allergens (apple, rMal d 1; celery, rApi g 1; and carrot, rDau c 1),as well as natural pollen (birch and timothy grass) and plant food (apple,peach, kiwi, hazelnut, celery, and carrot) allergens, to identify cross-reactive allergens by using qualitative immunoblot inhibitions. In addition, we determined the percentage of plant food-specific IgE that can be preadsorbed with recombinant and natural pollen allergens by quantitative RAST inhibitions by using sera from 71 patients with GAS. Results: Preincubation of sera with recombinant and natural pollen allergens led to an almost complete inhibition of IgE binding to plant food allergens in Western blots, as well as in RAST inhibition experiments. In contrast, recombinant plant food allergens poorly inhibited IgE binding to Eet v 1. Conclusion: Most IgE epitopes in plant food recognized by patients with OAS are resembled by pollen allergens. Thus pollen allergens may be responsible for the elicitation and maintenance of GAS.

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Documento generato il 19/07/18 alle ore 13:17:11