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Titolo:
DELETION OF C-MYB EXON-9 INDUCED BY INSERTION OF REPEATS
Autore:
SHINAGAWA T; ISHIGURO N; HORIUCHI M; MATSUI T; OKADA K; SHINAGAWA M;
Indirizzi:
OBIHIRO UNIV AGR & VET MED,DEPT VET PUBL HLTH OBIHIRO HOKKAIDO 080 JAPAN OBIHIRO UNIV AGR & VET MED,DEPT VET PUBL HLTH OBIHIRO HOKKAIDO 080 JAPAN OBIHIRO UNIV AGR & VET MED,DEPT VET PATHOL OBIHIRO HOKKAIDO 080 JAPAN IWATE UNIV,DEPT VET PATHOL MORIOKA IWATE 020 JAPAN
Titolo Testata:
Oncogene
fascicolo: 23, volume: 14, anno: 1997,
pagine: 2775 - 2783
SICI:
0950-9232(1997)14:23<2775:DOCEIB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING DOMAIN; T-LYMPHOMA-CELLS; V-MYB; TRANSCRIPTIONAL ACTIVATION; PROVIRAL INTEGRATION; CELLULAR PROGENITOR; ONCOGENE PRODUCT; GENE-EXPRESSION; LEUKEMIA-VIRUS; PROTEIN;
Keywords:
12-NUCLEOTIDE REPEAT; C-MYB; EXON SKIPPING; TRANSCRIPTION; T LYMPHOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
T. Shinagawa et al., "DELETION OF C-MYB EXON-9 INDUCED BY INSERTION OF REPEATS", Oncogene, 14(23), 1997, pp. 2775-2783

Abstract

A simple repeat was found to be inserted into exon 9 of the c-myb gene in three out of 20 bovine T lymphomas. The repeat was composed of multiple copies of a 12-nucleotide motif and had no significant homologyto the sequences reported so far. Tumor cells containing the repeat expressed two kinds of c-myb mRNA: (1) are that included the repetitivesequence in exon 9, and (2) are that lacked the whole sequence of exon 9. Transfection of an expression vector containing exon and intron sequences and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of the mRNA demonstrated that the insertion of the repeat enhanced exon skipping of the transfected minigene. These observations imply that the insertion of the repeat may enhance exon skipping of the c-myb pre-mRNA. Although the transcription-activating activity by the c-Myb with the repeat was low, that by the c-Myb without exon 9 was three- to eightfold higher than the wild-type c-Myb. These data suggest that insertion of the 12-nucleotide repeat in codon 359 may result in c-Myb proteins having high- and low-transcription-activating activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:25:09