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Titolo:
Reactions of platinum(II) complexes with a DNA hairpin, d(CGCGTTGTTCGCG): structural characterization and kinetic studies
Autore:
Meroueh, M; Kjellstrom, J; Martensson, KSM; Elmroth, SKC; Chow, CS;
Indirizzi:
Univ Lund, Ctr Chem, SE-22100 Lund, Sweden Univ Lund Lund Sweden SE-22100 niv Lund, Ctr Chem, SE-22100 Lund, Sweden Wayne State Univ, Dept Chem, Detroit, MI 48202 USA Wayne State Univ Detroit MI USA 48202 v, Dept Chem, Detroit, MI 48202 USA
Titolo Testata:
INORGANICA CHIMICA ACTA
fascicolo: 1-2, volume: 297, anno: 2000,
pagine: 145 - 155
SICI:
0020-1693(200001)297:1-2<145:ROPCWA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTICANCER DRUG CISPLATIN; INTRASTRAND CROSS-LINK; AQUEOUS-SOLUTION; MAJOR ADDUCT; BINDING; NMR; OLIGONUCLEOTIDES; PLATINATION; TRANS-DIAMMINEDICHLOROPLATINUM(II); CIS-DIAMMINEDICHLOROPLATINUM(II);
Keywords:
cisplatin; hairpin; kinetics; DNA conformation; chemical probing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Elmroth, SKC Univ Lund, Ctr Chem, POB 124, SE-22100 Lund, Sweden Univ LundPOB 124 Lund Sweden SE-22100 SE-22100 Lund, Sweden
Citazione:
M. Meroueh et al., "Reactions of platinum(II) complexes with a DNA hairpin, d(CGCGTTGTTCGCG): structural characterization and kinetic studies", INORG CHIM, 297(1-2), 2000, pp. 145-155

Abstract

Reactions of the platinum complexes cis-[Pt(NH3)(2)Cl(OH2)](+) (1) and cis-[Pt(NH3)(NH2C6H11)Cl(OH2)](+) (2) with a DNA hairpin, d(CGCGTTGTTCGCG) (DNA I), were investigated as a function of pH and ionic strength. The rates of platination were monitored by HPLC. The sites of reactivity for complexes1 and 2 were determined by chemical probing with dimethylsulfate (DMS), hydrazine, and Fe(EDTA)(2-). The effects of platination on DNA structure and stability were determined by using gel electrophoresis, circular dichroism (CD), and thermal melting. In general, the platinum complexes were most reactive at G7 in the hairpin loop, they caused a destabilization of the DNA hairpin, and they caused a B- to Z-form transition in the DNA conformation. (C) 2000 Elsevier Science S.A. All rights reserved.

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Documento generato il 30/09/20 alle ore 05:26:40