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Titolo:
Stable cation coordination at a single outer pore residue defines permeation properties in Kir channels
Autore:
Wischmeyer, E; Doring, F; Karschin, A;
Indirizzi:
Max Planck Inst Biophys Chem, Dept Mol Neurobiol Signal Transduct, D-37070Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-37070ottingen, Germany
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 466, anno: 2000,
pagine: 115 - 120
SICI:
0014-5793(20000121)466:1<115:SCCAAS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECTIFYING POTASSIUM CHANNEL; RECTIFIER K+ CHANNEL; SELECTIVITY FILTER; IRK1; MUTAGENESIS; MUTATIONS; REGION; NERVE;
Keywords:
inwardly rectifying K channel; Kir7.1; Kir2.1; ion selectivity; anomalous mole fraction; multi-ion pore;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Karschin, A Max Planck Inst Biophys Chem, Dept Mol Neurobiol Signal Transduct, Fassberg 11, D-37070 Gottingen, Germany Max Planck Inst Biophys Chem Fassberg 11 Gottingen Germany D-37070
Citazione:
E. Wischmeyer et al., "Stable cation coordination at a single outer pore residue defines permeation properties in Kir channels", FEBS LETTER, 466(1), 2000, pp. 115-120

Abstract

In epithelial Kir7.1 channels a non-conserved methionine in the outer poreregion adjacent to the G-Y-G selectivity filter (position +2) was found todetermine unique properties for permeant and blocking ions characteristic of a K+ channel in a single-occupancy state. The monovalent cation permeability sequence of Kir7,1 channels expressed in Xenopus oocytes was T1(+) > K > Rb+ > NH4+ > Cs+ > Na+ > Li+, but the macroscopic conductance for Rb+ was similar to 8-fold larger than for the smaller K+ ions, and decreased similar to 40-fold with the conserved arginine at the +2 position (Kir7.1M125R). Moreover, in Kir7.1 Rb+ restored the typical permeation properties of other multi-ion channels indicating that a stable coordination of permeant ions at the +2 position defines the initial step in the conduction pathway ofKir channels. (C) 2000 Federation of European Biochemical Societies.

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Documento generato il 22/09/20 alle ore 11:04:50