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Titolo:
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY
Autore:
Toba, K; Koike, T; Watanabe, K; Fuse, I; Takahashi, M; Hashimoto, S; Takahashi, H; Abe, T; Yano, T; Shibazaki, Y; Itoh, H; Aizawa, Y;
Indirizzi:
Niigata Univ, Sch Med, Dept Internal Med 1, Niigata 9518520, Japan NiigataUniv Niigata Japan 9518520 nternal Med 1, Niigata 9518520, Japan
Titolo Testata:
EUROPEAN JOURNAL OF HAEMATOLOGY
fascicolo: 1, volume: 64, anno: 2000,
pagine: 10 - 21
SICI:
0902-4441(200001)64:1<10:CKSONH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; ACTIVATED G1 LYMPHOCYTES; FLOW CYTOMETRIC ANALYSIS; ACUTE MYELOID-LEUKEMIA; DNA CONTENT; LYMPHOKINE REGULATION; LINEAGE INFIDELITY; SURFACE PHENOTYPE; PROGENITOR CELLS; GROWTH-FACTORS;
Keywords:
normal hematopoiesis; acute leukemia; DNA/RNA quantification; 7AAD/PY; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Toba, K Niigata Univ, Sch Med, Dept Internal Med 1, Asahimachi Dori 1, Niigata 9518520, Japan Niigata Univ Asahimachi Dori 1 Niigata Japan 9518520 18520, Japan
Citazione:
K. Toba et al., "Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY", EUR J HAEMA, 64(1), 2000, pp. 10-21

Abstract

We have used the 7AAD/PY method to analyze the cell cycle status of normalhuman bone marrow hematopoiesis, and found that the cell kinetics differed. There were cells with relatively low levels of RNA in the S-phase (Type I) and a high level in the S-phase (Type II). T-cells, B-cells, nucleated red cells and CD34(+)/CD19(+) early B-cells in bone marrow were Type I, whereas myelomonocytic subset and CD34(+)/CD33-dim(+) common myeloid cells were Type II. AC133(+)/CD38-dim cells, which were thought to be lineage-marker negative hematopoietic stem cells, had intermediate amounts of RNA in the S-phase between Type I and II (Type 0). Seventy-four cases of acute leukemia were also analyzed. Most of the T- and B-ALL cases were found to be Type I,most of the ANLL cases were Type II, and there were 10 cases that were Type 0. These findings yielded fundamental information about normal hematopoiesis and acute leukemia.

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Documento generato il 29/03/20 alle ore 11:49:45