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Titolo:
Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains
Autore:
Smits, THM; Rothlisberger, M; Witholt, B; van Beilen, JB;
Indirizzi:
ETH Honggerberg, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Honggerberg Zurich Switzerland CH-8093 , CH-8093 Zurich, Switzerland
Titolo Testata:
ENVIRONMENTAL MICROBIOLOGY
fascicolo: 4, volume: 1, anno: 1999,
pagine: 307 - 317
SICI:
1462-2912(199908)1:4<307:MSFAHG>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYMATIC OMEGA-OXIDATION; NUCLEOTIDE-RUBREDOXIN REDUCTASE; PSEUDOMONAS-OLEOVORANS; ACINETOBACTER-CALCOACETICUS; ESCHERICHIA-COLI; XYLENE MONOOXYGENASE; CLONING VECTORS; ALKBAC OPERON; NUCLEIC-ACID; N-ALKANES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: van Beilen, JB ETH Honggerberg, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Honggerberg Zurich Switzerland CH-8093 h, Switzerland
Citazione:
T.H.M. Smits et al., "Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains", ENVIRON MIC, 1(4), 1999, pp. 307-317

Abstract

We have developed highly degenerate oligonucleotides for polymerase chain reaction (PCR) amplification of genes related to the Pseudomonas oleovoransGPo1 and Acinetobacter sp. ADP1 alkane hydroxylases, based on a number of highly conserved sequence motifs. In all Gram-negative and in two out of three Gram-positive strains able to grow on medium- (C-6-C-11) Or long-chain n-alkanes (C-12-C-16), PCR products Of the expected size were obtained. ThePCR fragments were cloned and sequenced and found to encode peptides with 43.2-93.8% sequence identity to the corresponding fragment of the P. oleovorans GPo1 alkane hydroxyiase. Strains that were unable to grow on n-alkanesdid not yield non products with homology to alkane hydroxylase genes. The alkane hydroxylase genes of Acinetobacter calcoaceticus EB104 and Pseudomonas putida P1 were cloned using the PCR products as probes. The two genes allow an alkane hydroxylase-negative mutant of Acinetobacter sp. ADP1 and an Escherichia coil recombinant containing all P. oleovorans alk genes except alkB, respectively, to grow on n-alkanes, showing that the cloned genes do indeed encode alkane hydroxylases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:30:26