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Titolo:
Localisation of P-2x receptors in human salivary gland epithelial cells and human embryonic kidney cells by sodium dodecyl sulfate-polyacrylamide gelelectrophoresis/Western blotting and immunofluorescence
Autore:
Worthington, RA; Dutton, JL; Poronnik, P; Bennett, MR; Barden, JA;
Indirizzi:
Univ Sydney, Dept Anat & Histol, Inst Biomed Res, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 ed Res, Sydney, NSW 2006, Australia Univ Sydney, Dept Physiol, Sydney, NSW 2006, Australia Univ Sydney SydneyNSW Australia 2006 hysiol, Sydney, NSW 2006, Australia
Titolo Testata:
ELECTROPHORESIS
fascicolo: 10, volume: 20, anno: 1999,
pagine: 2065 - 2070
SICI:
0173-0835(199907)20:10<2065:LOPRIH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION-CHANNEL; P2X RECEPTORS; SENSORY NEURONS; EXTRACELLULAR ATP; RAT; INHIBITION; BRAIN; LINE; IDENTIFICATION; PURINOCEPTOR;
Keywords:
P-2x receptors; human salivary gland epithelial cells; antibody; human embryonic kidney cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Barden, JA Univ Sydney, Dept Anat & Histol, Inst Biomed Res, F13, Sydney, NSW 2006, Australia Univ Sydney F13 Sydney NSW Australia 2006 , NSW 2006, Australia
Citazione:
R.A. Worthington et al., "Localisation of P-2x receptors in human salivary gland epithelial cells and human embryonic kidney cells by sodium dodecyl sulfate-polyacrylamide gelelectrophoresis/Western blotting and immunofluorescence", ELECTROPHOR, 20(10), 1999, pp. 2065-2070

Abstract

Human salivary gland epithelial cells, a continuous cell line derived froman irradiated human salivary gland and human embryonic kidney cell line human embryonic kidney (HEK)293 were examined for the purpose of establishingwhether they expressed endogenous P-2X ionotropic receptors at any stage in their cycles. HSG cells were found to express P2X1-6 subtypes using both Western blotting and immunofluorescence labeling. HEK293 cells had no detectable levels of P2X1-3 and P-2X6 under normal circumstances along with verylow levels of P-2X4 and P-2X5 but when the cells were grown past confluence then all subtypes were expressed on the surface membrane with the exception of P-2X2. The results are discussed in terms of the likely influence of ATP acting as an intercellular signaling molecule.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:39:01