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Titolo:
The protein composition of ferret parotid saliva as revealed by high-resolution electrophoretic methods
Autore:
Williams, KM; Ekstrom, J; Marshall, T;
Indirizzi:
Univ Sunderland, Sch Sci, Analyt Biochem Res Grp, Sunderland SR1 3RD, Durham, England Univ Sunderland Sunderland Durham England SR1 3RD R1 3RD, Durham, England Univ Gothenburg, Dept Pharmacol, Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden g, Dept Pharmacol, Gothenburg, Sweden
Titolo Testata:
ELECTROPHORESIS
fascicolo: 14, volume: 20, anno: 1999,
pagine: 2818 - 2823
SICI:
0173-0835(199910)20:14<2818:TPCOFP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; PARASYMPATHETIC NERVE-STIMULATION; GENE-RELATED PEPTIDE; SUBSTANCE-P; SECRETION; RAT; GLANDS; DEPLETION; ATROPINE;
Keywords:
ferret parotid saliva; salivary proteins; two-dimensional electrophoresis; silver staining; sodium dodecyl sulfate - polyacrylamide gel electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Marshall, T Univ Sunderland, Sch Sci, Analyt Biochem Res Grp, Sunderland SR1 3RD, Durham, England Univ Sunderland Sunderland Durham England SR1 3RD am, England
Citazione:
K.M. Williams et al., "The protein composition of ferret parotid saliva as revealed by high-resolution electrophoretic methods", ELECTROPHOR, 20(14), 1999, pp. 2818-2823

Abstract

Ferret parotid saliva has been analysed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE) to determine, for the first time, its protein composition. SDS-PAGE, in combination with Coomassie Brilliant Blue (CBB) staining, revealed upto 20 bands and the patterns were characterised by major protein constituents of M-r 105 000, 51 000, 47 000, 33 000, 22 000 and 16 400 common to allsamples from all animals. Sequential samples collected from the same animal during prolonged stimulation of the parasympathetic nerve (40 min at 40 Hz) showed subtle but reproducible protein changes. Saliva collected from different animals varied widely in the amount of a protein M-r 66 000. 2-DE, in combination with silver staining, revealed up to 300 spots and the patterns were characterised by major protein constituents of M-r 105 000 (p/6.3-7.2), M-r 66 000 (p/4.7-5.3), M-r 51 000 (p/5.0-5.7), M-r 47 000 (p/6.0-7.5), and M-r 33 000 (p/4.7-6.0). Many of the polypeptide spot clusters consisted of one or more horizontal strings of spots suggesting extensive microheterogeneity. Both SDS-PAGE and 2-DE indicated that the protein patterns of ferret parotid saliva evoked by electrical stimulation of the parasympathetic nerve in the absence or presence of atropine are similar, i.e., the protein composition of the atropine-resistant nonadrenergic, noncholinergic (NANC) secretion is similar to that of saliva evoked in the absence of muscarinic receptor blockade.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 05:36:37