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Titolo:
Monoclonal antibody against a neuronal antigen impairs formation of the axon scaffold in grasshopper central nervous system
Autore:
von Bernhardi, R; Carpenter, EM;
Indirizzi:
Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Biol, Salt Lake City, UT 84112 USA
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 6, volume: 21, anno: 1999,
pagine: 423 - 435
SICI:
0378-5866(199911/12)21:6<423:MAAANA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULES; GROWTH CONES; DEVELOPMENTAL EXPRESSION; IMMUNOGLOBULIN SUPERFAMILY; HORSERADISH-PEROXIDASE; EMBRYONIC-DEVELOPMENT; SULFATED GLYCOLIPIDS; NEURITE OUTGROWTH; OLFACTORY SYSTEM; FASCICLIN-II;
Keywords:
CNS development; axonal guidance; neurite outgrowth; cell adhesion; pioneer neurons; glycolipid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: von Bernhardi, R Univ Los Andes, Sch Med, San Carlos Apoquindo 2200, Santiago, Chile Univ Los Andes San Carlos Apoquindo 2200 Santiago Chile
Citazione:
R. von Bernhardi e E.M. Carpenter, "Monoclonal antibody against a neuronal antigen impairs formation of the axon scaffold in grasshopper central nervous system", DEV NEUROSC, 21(6), 1999, pp. 423-435

Abstract

The pathway a growing axon follows is determined by a number of cues, including differential adhesion to surface molecules on axons and the matrix ofthe fascicles a long wh ich they grow. We have characterized the differential expression of an extracellular antigen and the effects of a monoclonal antibody against this molecule on the development of the grasshopper central nervous system (CNS). The 5C1 monoclonal antibody was generated against ganglion chains of grasshopper embryos; it labels cell bodies of newly differentiated neurons and their axons as they extend. Electron microscopy of embryos at 42% of development reveals that 5C1 labels neuronal filopodia, axons and somata, and areas of glial membrane in apposition to neurite fascicles. After 70% of development, labeling is lost from axon bundles, but remains on cell bodies. 5C1 also cross-reacts with an epitope expressed in Drosophila CNS during embryonic development. Enzymatic digestion suggests that the antigen recognized by the antibody is likely to be a glycolipid. In embryos exposed to 5C1 during early stages of development of the CNS, at the time when the fi rst axons begin to extend, the formation of axon pathways is blocked or greatly delayed. Our results suggest that the 5C1 antigen participates in the formation of the axon scaffold and may play a functional role in the initiation and maintenance of axon outgrowth during early development of the CNS. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:29:43