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Titolo:
Androgen receptor expression is greater in macrophages from male than fromfemale donors - A sex difference with implications for atherogenesis
Autore:
McCrohon, JA; Death, AK; Nakhla, S; Jessup, W; Handelsman, DJ; Stanley, KK; Celermajer, DS;
Indirizzi:
Royal Prince Alfred Hosp, Dept Cardiol, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Sydney NSW Australia 2050 y, NSW 2050, Australia Royal Prince Alfred Hosp, Heart Res Inst, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Sydney NSW Australia 2050 y, NSW 2050, Australia Univ Sydney, Dept Med, Sydney, NSW 2006, Australia Univ Sydney Sydney NSWAustralia 2006 pt Med, Sydney, NSW 2006, Australia
Titolo Testata:
CIRCULATION
fascicolo: 3, volume: 101, anno: 2000,
pagine: 224 - 226
SICI:
0009-7322(20000125)101:3<224:AREIGI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; ATHEROSCLEROSIS; ACCUMULATION; DISEASE; CELLS;
Keywords:
atherosclerosis; cells; hormones;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Celermajer, DS Royal Prince Alfred Hosp, Dept Cardiol, Missenden Rd, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Missenden Rd Sydney NSW Australia 2050
Citazione:
J.A. McCrohon et al., "Androgen receptor expression is greater in macrophages from male than fromfemale donors - A sex difference with implications for atherogenesis", CIRCULATION, 101(3), 2000, pp. 224-226

Abstract

background sex is an independent risk factor for the extent and severity of atherosclerosis. The influence of androgens on foam cell formation, a keyevent in atherogenesis, has not yet been investigated. Methods and Results-Primary human monocytes were allowed to differentiate into macrophages. RNA was then extracted from healthy male-donor (n=8) and premenopausal female-donor (n=8) macrophages, and message for the androgen receptor (AR) was examined by RT-PCR. There was a significantly higher level of AR mRNA in macrophages isolated from men than in those from women (0.64+/-0.06 versus 0.15+/-0.02 amol/mu g total RNA; P<0.001). AR mRNA levels were similar in macrophages from postmenopausal and premenopausal women (P=0.16). The functional consequence of this sex difference was then explored, Lipid-loading studies were performed on male (n=9) macrophages treated withthe androgen dihydrotestosterone (DHT) and/or the AR antagonist hydroxyflutamide. These showed that DHT caused a dose-dependent and receptor-mediatedincrease in macrophage cholesteryl ester content (109+/-10%, 117+/-3%, and120+/-4% for 4, 40, and 400 nmol/L DHT, respectively, as a percentage of control, P=0.002; 95+/-8% for DHT with hydroxyflutamide, P=0.58 versus controls). By contrast, then was no significant effect of androgen on lipid lending in female-donor macrophages (P>0.2 versus controls). Conclusions-Sex differences in androgen-mediated macrophage lipid lending may contribute to the greater prevalence and severity of atherosclerosis inmen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:00:25