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Titolo:
Nuclear matrix proteins associated with DNA in situ in hormone-dependent and hormone-independent human breast cancer cell lines
Autore:
Spencer, VA; Samuel, SK; Davie, JR;
Indirizzi:
Univ Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada Univ Manitoba Winnipeg MB Canada R3E 0V9 ol, Winnipeg, MB R3E 0V9, Canada Univ Manitoba, Dept Biochem & Mol Biol, Winnipeg, MB R3E 0V9, Canada Univ Manitoba Winnipeg MB Canada R3E 0V9 ol, Winnipeg, MB R3E 0V9, Canada
Titolo Testata:
CANCER RESEARCH
fascicolo: 2, volume: 60, anno: 2000,
pagine: 288 - 292
SICI:
0008-5472(20000115)60:2<288:NMPAWD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; EXPERIMENTAL COEXPRESSION; TRANSCRIPTION FACTORS; CROSS-LINKING; IN-SITU; ORGANIZATION; CHROMATIN; VIMENTIN; ATTACHMENT; PHENOTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Davie, JR Univ Manitoba, Manitoba Inst Cell Biol, 100 Olivia St, Winnipeg,MB R3E 0V9, Canada Univ Manitoba 100 Olivia St Winnipeg MB Canada R3E 0V9 9, Canada
Citazione:
V.A. Spencer et al., "Nuclear matrix proteins associated with DNA in situ in hormone-dependent and hormone-independent human breast cancer cell lines", CANCER RES, 60(2), 2000, pp. 288-292

Abstract

The nuclear matrix is a dynamic RNA-protein complex that organizes chromatin and regulates nuclear DNA metabolism. Nuclear matrix proteins informative in the diagnosis of cancer have been identified. Here, the nuclear matrixbreast cancer proteins (NMBCs) cross-linked to nuclear DNA in situ with cisplatin in human breast cancer cell lines were analyzed by two-dimensional gel electrophoresis. We identified NMBCs that were differentially associated with nuclear DNA of hormone-dependent and -independent breast cancer celllines. Three DNA cross-linked NMBCs were found to be exclusive to estrogenreceptor-positive, hormone-dependent breast cancer cells, whereas two NMBCs were observed only in estrogen receptor-negative, hormone-independent breast cancer cells. Changes in these NMBCs were observed when hormone-dependent breast cancer cells became hormone independent. Furthermore, we show that the intermediate filament protein vimentin is associated with the nuclearDNA of MDA-MB-231 breast cancer cells, an estrogen receptor-negative, hormone-independent breast cancer cell line with high metastatic potential. These nuclear matrix DNA-binding proteins may play important roles in breast tumorigenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:34:07