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Titolo:
Spontaneous apoptosis in ovarian carcinomas: a positive association with p53 gene mutation is dependent on growth fraction
Autore:
Kupryjanczyk, J; Dansonka-Mieszkowska, A; Szymanska, T; Karpinska, G; Rembiszewska, A; Rusin, M; Konopinski, R; Kraszewska, E; Timorek, A; Yandell, DW; Stelmachow, J;
Indirizzi:
Marie Sklodowska Curie Mem Canc Ctr, Dept Mol Biol, PL-02781 Warsaw, Poland Marie Sklodowska Curie Mem Canc Ctr Warsaw Poland PL-02781 arsaw, Poland Marie Sklodowska Curie Mem Canc Ctr, Dept Biostat, PL-02781 Warsaw, PolandMarie Sklodowska Curie Mem Canc Ctr Warsaw Poland PL-02781 arsaw, Poland Inst Oncol, PL-02781 Warsaw, Poland Inst Oncol Warsaw Poland PL-02781Inst Oncol, PL-02781 Warsaw, Poland Dept Tumour Biol, PL-4100 Gliwice, Poland Dept Tumour Biol Gliwice Poland PL-4100 ur Biol, PL-4100 Gliwice, Poland Brodnowski Hosp, Dept Pathol, Med Acad Warsaw, PL-03242 Warsaw, Poland Brodnowski Hosp Warsaw Poland PL-03242 d Warsaw, PL-03242 Warsaw, Poland Brodnowski Hosp, Dept Obstet & Gynecol, Med Acad Warsaw, PL-03242 Warsaw, Poland Brodnowski Hosp Warsaw Poland PL-03242 d Warsaw, PL-03242 Warsaw, Poland Univ Vermont, Coll Med, Dept Pathol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 ept Pathol, Burlington, VT 05405 USA Vermont Canc Ctr, Burlington, VT 05405 USA Vermont Canc Ctr Burlington VTUSA 05405 nc Ctr, Burlington, VT 05405 USA
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 3, volume: 82, anno: 2000,
pagine: 579 - 583
SICI:
0007-0920(200002)82:3<579:SAIOCA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR P53; P53-DEPENDENT APOPTOSIS; PROTEIN ACCUMULATION; CELL-DEATH; BAX GENE; EXPRESSION; BCL-2; CANCER; CYTOTOXICITY; ARREST;
Keywords:
apoptosis; p53 gene mutation; p53 protein accumulation; growth fraction; bcl-2; ovarian cancer; survival;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kupryjanczyk, J Marie Sklodowska Curie Mem Canc Ctr, Dept Mol Biol, Roentgena 5, PL-02781 Warsaw, Poland Marie Sklodowska Curie Mem Canc Ctr Roentgena 5 Warsaw Poland PL-02781
Citazione:
J. Kupryjanczyk et al., "Spontaneous apoptosis in ovarian carcinomas: a positive association with p53 gene mutation is dependent on growth fraction", BR J CANC, 82(3), 2000, pp. 579-583

Abstract

Changes in cell survival contribute to tumour development, influence tumour biology and its response to chemotherapy. p53 gene alterations should negatively affect apoptosis by impaired p53-dependent apoptotic response. We looked for associations between spontaneous apoptosis, p53 gene mutation, p53 protein accumulation, growth fraction, bcl-2 expression and histological parameters in 64 ovarian, four tubal and three peritoneal carcinomas. Apoptotic cells were detected with the TUNEL method. p53 gene Variants were detected by the single-strand conformation polymorphism and were sequenced directly. P53, Ki-67 and bcl-2 protein expressions were detected immunohistochemically. A weighed multiple logistic regression model was applied. Apoptotic index (Al) ranged 0.02-0.18 (mean 0.11); proliferation index (PI) ranged 3-90% (mean 54%). p53 gene mutations were present in 51, p53 protein accumulation in 46, and diffuse bcl-2 expression in 29 of 71 tumours. The Al was positively associated with the presence of p53 gene mutation (P = 0.011). However, the PI included into the analysis did positively influence the Al (P = 0.02) and diminished the association with p53 gene mutation (P = 0.082). The Al was negatively associated with good histological differentiation (P = 0.0006), the serous tumour type (P = 0.002), and diffuse bcl-2 expression (P = 0.025). Strong bcl-2 expression was associated with endometrioid tumour type (P = 0.002). FIGO stage and p53 protein accumulation were the only parameters that influenced overall survival time. Thus, our results suggest that histological tumour type and grade are major determinants of spontaneous apoptosis in ovarian carcinomas; p53 alterations do not adversely butrather positively affect spontaneous apoptosis by increasing growth fraction. This, in turn, suggests p53-independency of spontaneous apoptosis in ovarian carcinomas. (C) 2000 Cancer Research Campaign.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 16:09:40