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Titolo:
A NEW METHOD FOR THE RAPID MEASUREMENT OF CHOLESTEROL CRYSTALLIZATIONIN MODEL BILES USING A SPECTROPHOTOMETRIC MICROPLATE READER
Autore:
SOMJEN GJ; RINGEL Y; KONIKOFF FM; ROSENBERG R; GILAT T;
Indirizzi:
ICHILOV HOSP,SOURASKI MED CTR,DEPT GASTROENTEROL IL-64239 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER FAC MED,MINERVA CTR CHOLESTEROL GALLSTONES & LIPID METAB IL-69978 TEL AVIV ISRAEL
Titolo Testata:
Journal of lipid research
fascicolo: 5, volume: 38, anno: 1997,
pagine: 1048 - 1052
SICI:
0022-2275(1997)38:5<1048:ANMFTR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GALLBLADDER BILE; GALLSTONE FORMATION; CRYSTAL-GROWTH; NUCLEATION; ASSAY;
Keywords:
PHOSPHOLIPIDS; BILE SALTS; NUCLEATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Somjen et al., "A NEW METHOD FOR THE RAPID MEASUREMENT OF CHOLESTEROL CRYSTALLIZATIONIN MODEL BILES USING A SPECTROPHOTOMETRIC MICROPLATE READER", Journal of lipid research, 38(5), 1997, pp. 1048-1052

Abstract

Measurements of the cholesterol crystal observation time, and particularly the crystal growth rate in model biles, are important in biliarypathophysiology. The aim of this study was to develop a semi-automated method permitting multiple, simultaneous, and precise measurements of the crystal growth rate in model biles. Incubated model biles were mixed with a high concentration of NaTDC to solubilize noncrystalline turbidity and spectrophotometric measurements were performed. In parallel, samples were observed by light microscopy. The absorbance correlated linearly with the crystal mass and permitted quantitation of the crystal growth rate. Polarized light microscopy was more sensitive than spectrophotometry for determining the initial crystal observation time, while spectrophotometry was more precise and quantitative for measuring the crystal growth rate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:40:42