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Titolo:
Detection and imaging of L-glutamate released from mouse-brain slices withan enzyme-based membrane
Autore:
Hirano, A; Asakawa, M; Kido, N; Sugawara, M;
Indirizzi:
Nihon Univ, Coll Humanities & Sci, Dept Chem, Tokyo 1568550, Japan Nihon Univ Tokyo Japan 1568550 es & Sci, Dept Chem, Tokyo 1568550, Japan
Titolo Testata:
ANALYTICAL SCIENCES
fascicolo: 1, volume: 16, anno: 2000,
pagine: 25 - 29
SICI:
0910-6340(200001)16:1<25:DAIOLR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NMDA RECEPTOR CHANNEL; CULTURED NERVE-CELLS; FLUORESCENT INDICATORS; CALCIUM ACCUMULATION; ONLINE SENSOR; MICROSCOPY; DEPLETION; PROBES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, M Nihon Univ, Coll Humanities & Sci, Dept Chem, Tokyo 1568550, Japan Nihon Univ Tokyo Japan 1568550 pt Chem, Tokyo 1568550, Japan
Citazione:
A. Hirano et al., "Detection and imaging of L-glutamate released from mouse-brain slices withan enzyme-based membrane", ANAL SCI, 16(1), 2000, pp. 25-29

Abstract

A new and simple method for detecting and color imagine of L-glutamate released in mouse-brain slices is described based on an enzyme-coupling reaction. The method utilizes L-glutamate oxidase (GluOx) and horseradish peroxidase (HRP) immobilized in a bovine serum albumin (BSA)-glutaraldehyde matrixon a poly-L-lysine-coated glass cover slip. The enzymes are spontaneously and continuously supplied from the membrane into an extracellular solution of the brain slice placed on the membrane after dipping in a redox substrate (DA-64). The Bindschedler's Green (BG) converted from the DA-64 by the enzyme reaction gives green signals in the brain slice. which are related to the concentration of L-glutamate released in its regions. The first examples of color images for the spatial distribution of L-glutamate in the regions of adult mouse brain slices are demonstrated. The regions where the intense green signals were observed matched the reported distribution of mRNAs encoding the NMDA receptor in the mouse-brain slice.

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Documento generato il 25/01/20 alle ore 16:00:05