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Titolo:
Postpartum umbilical cord blood collection for transplantation: A comparison of three methods
Autore:
Elchalal, U; Fasouliotis, SJ; Shtockheim, D; Brautbar, C; Schenker, JG; Weinstein, D; Nagler, A;
Indirizzi:
Hadassah Univ Hosp, Dept Obstet & Gynecol, IL-91120 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91120 IL-91120 Jerusalem, Israel Hadassah Univ Hosp, Lautenberg Ctr Tissue Typing, IL-91120 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91120 IL-91120 Jerusalem, Israel Hadassah Univ Hosp, Human Umbil Cord Blood Bank & Bone Marrow Transpl, IL-91120 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91120 IL-91120 Jerusalem, Israel
Titolo Testata:
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
fascicolo: 1, volume: 182, anno: 2000,
parte:, 1
pagine: 227 - 232
SICI:
0002-9378(200001)182:1<227:PUCBCF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; UNRELATED DONORS; PROGENITOR CELLS; PLACENTAL-BLOOD; CRYOPRESERVATION; RECONSTITUTION; SEPARATION; RECIPIENTS; BANKING;
Keywords:
collection methods; human umbilical cord blood; transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Elchalal, U Hadassah Med Org, Dept Obstet & Gynecol, POB 12000, IL-91120 Jerusalem, Israel Hadassah Med Org POB 12000 Jerusalem Israel IL-91120 , Israel
Citazione:
U. Elchalal et al., "Postpartum umbilical cord blood collection for transplantation: A comparison of three methods", AM J OBST G, 182(1), 2000, pp. 227-232

Abstract

OBJECTIVE: This study was undertaken to compare 3 methods of collection ofhuman umbilical cord blood. STUDY DESIGN: Seventy-five women with uncomplicated vaginal deliveries were divided equally into 3 groups. One of 3 cord blood collection methods wasapplied to each woman. Method 1 was collection of cord blood into a standard donation blood bag. Methods 2 and 3 used a syringe to perform a sodium chloride solution flush and drain, which included withdrawal of cord blood by a syringe until the delivery of the placenta, followed by flushing through a catheter one of the umbilical arteries with sodium chloride solution and collection of the cord blood either into an open sterile container (method 2) or into a standard donation blood bag (method 3). Analyses included comparisons among the 3 groups of volume collected, total number of white blood cells, and bacterial contamination rates (positive culture results). In addition a correlation was made between the different variables and the collected cord blood nucleated cells. RESULTS: Cord blood collection by the blood bag method (method 1), which is presently the standard clinical practice, resulted in a mean blood volumeof 76.4 +/- 32.1 mL and a mean total white blood cell count of 835 +/- 507x 10(6) cells. With collection methods 2 and 3, in which as much blood as possible was withdrawn by syringe white the placenta was still in utero followed by a second collection after infusion of the umbilical artery with sodium chloride solution, the mean volume collected was significantly higher (P <.05) at 174.4 +/- 42.8 mL and 173.7 +/- 41.3 mL, respectively, with significantly higher (P <.001) mean total white blood cell counts of 1624 +/- 887 x 10(6) cells and 1693 +/- 972 x 10(6) cells, respectively. A direct correlation was observed between the cord blood volume collected and placental weight, whereas no correlations were observed with maternal age. pregnancy duration, or the neonate's weight. Bacterial contamination was significantly higher (P=.04) in cord blood collections obtained by method 2 (48%) than by methods 1 (16%) and 3 (19%). CONCLUSIONS: The syringe-assisted sodium chloride solution flush collection method with a blood bag (method 3) was found to be the most effective method for human umbilical cord blood collection. This method doubles the total white blood cells collected with respect to current yields, which may make cord blood transplantation applicable for adults.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/08/20 alle ore 10:40:53