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Titolo:
The APHA standard method for the enumeration of somatic coliphages in water has low efficiency of plating
Autore:
Green, J; Bruce, K; Conner, C; Mutesi, R;
Indirizzi:
Napier Univ, Dept Biol Sci, Edinburgh EH10 5DT, Midlothian, Scotland Napier Univ Edinburgh Midlothian Scotland EH10 5DT , Midlothian, Scotland
Titolo Testata:
WATER RESEARCH
fascicolo: 3, volume: 34, anno: 2000,
pagine: 759 - 762
SICI:
0043-1354(200002)34:3<759:TASMFT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DRINKING-WATER; BACTEROIDES-FRAGILIS; FECAL POLLUTION; PLAQUE-ASSAY; INDICATORS; BACTERIOPHAGES; ABUNDANCE; BACTERIA; VIRUSES; SEWAGE;
Keywords:
APHA standard method; somatic coliphages; e.o.p;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Green, J Napier Univ, Dept Biol Sci, 10 Colinton Rd, Edinburgh EH10 5DT, Midlothian, Scotland Napier Univ 10 Colinton Rd Edinburgh Midlothian Scotland EH10 5DT
Citazione:
J. Green et al., "The APHA standard method for the enumeration of somatic coliphages in water has low efficiency of plating", WATER RES, 34(3), 2000, pp. 759-762

Abstract

The standard method for the enumeration of somatic coliphages in water proposed by the American Public Health Association recommends the use of single layer plaque assays with 5 ml sample and the direct use of frozen and thawed host E. coil cultures. In assays of spiked river water the efficiency of plating (e.o.p.) of the standard method, using frozen/thawed host cultures, was significantly lower than comparable assays using unfrozen host cultures. Frozen/thawed cultures also produced significantly lower efficiency ofplating in scaled up versions of the standard method using both 50 and 100ml sample. The e.o.p. varied from 39.0-46.1% with frozen/thawed cultures and 67.7-74.4% with unfrozen cultures at OD 0.5, relative to overnight cultures. It is therefore recommended that overnight cultures are used in place of frozen/thawed cultures in the APHA standard method and scaled up versions of it. (C) 2000 Elsevier Science Ltd. All rights reserved.

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Documento generato il 01/04/20 alle ore 17:46:12