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Titolo:
Early direct and transneuronal effects in mice with targeted expression ofa toxin gene to D1 dopamine receptor neurons
Autore:
Padungchaichot, P; Wong, JYF; Natoli, AL; Massalas, JS; Finkelstein, DI; Lawrence, AL; Drago, J;
Indirizzi:
Monash Univ, Neurosci Grp, Dept Med, Monash Med Ctr, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Ctr, Clayton, Vic 3168, Australia Monash Univ, Dept Pharmacol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 acol, Clayton, Vic 3168, Australia
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 95, anno: 2000,
pagine: 1025 - 1033
SICI:
0306-4522(2000)95:4<1025:EDATEI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT STRIATAL NEURONS; SITE-SPECIFIC RECOMBINATION; SUBSTANTIA-NIGRA; GABA(A) RECEPTOR; MOLECULAR-CLONING; NEUROPEPTIDE-Y; PHENOTYPICAL CHARACTERIZATION; HUNTINGTONS-DISEASE; SUBUNIT EXPRESSION; EXCITOTOXIC INJURY;
Keywords:
knockout; neuropeptide Y; tyrosine hydroxylase; dopamine transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Drago, J Monash Univ, Neurosci Grp, Dept Med, Monash Med Ctr, Clayton Rd, Clayton, Vic 3168, Australia Monash Univ Clayton Rd Clayton Vic Australia 3168 3168, Australia
Citazione:
P. Padungchaichot et al., "Early direct and transneuronal effects in mice with targeted expression ofa toxin gene to D1 dopamine receptor neurons", NEUROSCIENC, 95(4), 2000, pp. 1025-1033

Abstract

The neurochemical profile was examined at postnatal day 3-4 in mutant micegenerated by in vivo Cre mediated activation of an attenuated diphtheria toxin gene inserted into the D1 dopamine receptor gene locus. An earlier study of this model had shown that D1 dopamine receptor, substance P and dynorphin were not expressed in the striatum. Quantitative in situ hybridizationanalysis showed an increase in D2 dopamine receptor and enkephalin messenger RNA expression. The nigrostriatal pathway in the mutant pups was intact with a normal number of dopaminergic neurons in the substantia nigra and the ventral tegmental area in addition to a normal pattern of striatal dopamine transporter and tyrosine hydroxylase immunoreactivity. Quantitative analysis of striatal dopamine transporter density using [H-3]mazindol showed a reduction of 26% suggesting a degree of transneuronal down-regulation. There was also a 49% reduction of striatal GABA receptor binding and a 36% reduction of striatal muscarinic receptor binding in mutant pups. The number ofhealthy striatal neuropeptide Y-containing interneurons was also substantially down-regulated in the mutant striatum. In contrast, there was an increase in the number of striatal cholinergic interneurons. Down-regulated cortical GABA receptor and muscarinic receptor binding was also observed in addition to subtle morphological changes in the neuropeptide Y-expressing population of cortical neurons. The changes reflect the early cascade of events which follows the ablationof D1 dopamine receptor-positive cells. Although extensive changes in a number of striatal and cortical neurons were demonstrated, only subtle transneuronal effects were seen in the nigrostriatal pathway. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:50:13