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Titolo:
Exon-intron organization of the human 5-HT3A receptor gene
Autore:
Bruss, M; Eucker, T; Gothert, M; Bonisch, H;
Indirizzi:
Univ Bonn, Inst Pharmacol & Toxicol, D-53113 Bonn, Germany Univ Bonn Bonn Germany D-53113 harmacol & Toxicol, D-53113 Bonn, Germany
Titolo Testata:
NEUROPHARMACOLOGY
fascicolo: 2, volume: 39, anno: 2000,
pagine: 308 - 315
SICI:
0028-3908(2000)39:2<308:EOOTH5>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION-CHANNEL; FUNCTIONAL EXPRESSION; 5-HYDROXYTRYPTAMINE(3) RECEPTOR; SPLICE VARIANTS; ANTICANCER THERAPY; MOLECULAR-CLONING; XENOPUS-OOCYTES; LIGAND-BINDING; SEROTONIN; SUBUNIT;
Keywords:
ligand-gated ion channel; 5-hydroxytryptamine(3) receptor; human gene structure; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bruss, M Univ Bonn, Inst Pharmacol & Toxicol, Reuterstr 2B, D-53113 Bonn, Germany Univ Bonn Reuterstr 2B Bonn Germany D-53113 -53113 Bonn, Germany
Citazione:
M. Bruss et al., "Exon-intron organization of the human 5-HT3A receptor gene", NEUROPHARM, 39(2), 2000, pp. 308-315

Abstract

The gene structure of the human 5-HT3A receptor gene was analyzed by exon to exon polymerase chain reaction and subsequent sequencing. The results were confirmed by restriction analysis and genomic Southern blotting. The coding region of the human gene was found to be split by eight introns at identical positions as in the murine 5-HT3A receptor gene. All exon-intron boundaries exhibited fully conserved splice donor and acceptor consensus sequences. The alternative splice acceptor in intron eight of the murine gene wasnot found in the human counterpart. The length of particular introns differs markedly from the murine gene. With the exception of intron 5, all humanintrons are longer than their murine counterparts. From the start to the stop codon the human gene stretches over about 14.5 kb. The human exon sequences confirm one of three published human 5-HT3A receptor cDNA sequences. Knowledge of the gene structure, including 1.9 kb of the 5' noncoding region, all introns and the exon-intron boundaries of the human 5-HT3A receptor gene should facilitate investigation of its potential role in psychiatric disorders. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:48:59