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Titolo:
Proper ascospore maturation requires the chs1(+) chitin synthase gene in Schizosaccharomyces pombe
Autore:
Arellano, M; Cartagena-Lirola, H; Hajibagheri, MAN; Duran, A; Valdivieso, MH;
Indirizzi:
Univ Salamanca, CSIC, Dept Genet & Microbiol, Inst Microbiol Bioquim, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 iol Bioquim, Salamanca 37007, Spain Imperial Canc Res Fund, Dept Electron Microscopy, London WC2A 3PX, EnglandImperial Canc Res Fund London England WC2A 3PX London WC2A 3PX, England
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 1, volume: 35, anno: 2000,
pagine: 79 - 89
SICI:
0950-382X(200001)35:1<79:PAMRTC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; CELL-WALL; MUTANTS; INVOLVEMENT; EXPRESSION; MORPHOGENESIS; LOCALIZATION; ARCHITECTURE; CATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Valdivieso, MH Univ Salamanca, CSIC, Dept Genet & Microbiol, Inst Microbiol Bioquim, Edificio Departamental, Salamanca 37007, Spain Univ Salamanca Edificio Departamental Salamanca Spain 37007
Citazione:
M. Arellano et al., "Proper ascospore maturation requires the chs1(+) chitin synthase gene in Schizosaccharomyces pombe", MOL MICROB, 35(1), 2000, pp. 79-89

Abstract

We have cloned chs1(+), a Schizosaccharomyces pombe gene with similarity to class II chitin synthases, and have shown that it is responsible for chitin synthase activity present in cell extracts from this organism. Analysis of this activity reveals that it behaves like chitin synthases from other fungi, although with specific biochemical characteristics. Deletion or overexpression of this gene does not lead to any apparent defect during vegetative growth. In contrast, chs1(+) expression increases significantly during sporulation, and this is accompanied by an increase in chitin synthase activity. In addition, spore formation is severely affected when both parental strains carry a chs1 deletion, as a result of a defect in the synthesis of the ascospore cell wall. Finally, we show that wild-type, but not chs1(-)/chs1(-), ascospore cell walls bind wheatgerm agglutinin. Our results clearly suggest the existence of a relationship between chs1(+), chitin synthesis and ascospore maturation in S. pombe.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 09:20:59