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Titolo:
Differentiation dependent activation of the myelin genes in purified oligodendrocytes is highly resistant to hypoglycemia
Autore:
Royland, JE; Konat, GW; Wiggins, RC;
Indirizzi:
W Virginia Univ, Sch Med, Dept Anat, Morgantown, WV 26505 USA W Virginia Univ Morgantown WV USA 26505 pt Anat, Morgantown, WV 26505 USA
Titolo Testata:
METABOLIC BRAIN DISEASE
fascicolo: 3, volume: 14, anno: 1999,
pagine: 189 - 195
SICI:
0885-7490(199909)14:3<189:DDAOTM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MALIC ENZYME-ACTIVITY; DEVELOPING RAT-BRAIN; UP-REGULATION; PROTEOLIPID PROTEIN; GLYCOPROTEIN GENE; CRITICAL PERIOD; MESSENGER-RNAS; FACTOR-ALPHA; GLIAL-CELLS;
Keywords:
oligodendrocyte cultures; glucose; gene expression; malic enzyme; membrane synthesis; myelination; undernutrition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Wiggins, RC W Virginia Univ, Sch Med, Dept Anat, POB 9128, Morgantown, WV 26505 USA W Virginia Univ POB 9128 Morgantown WV USA 26505 WV 26505 USA
Citazione:
J.E. Royland et al., "Differentiation dependent activation of the myelin genes in purified oligodendrocytes is highly resistant to hypoglycemia", METAB BRAIN, 14(3), 1999, pp. 189-195

Abstract

We have previously demonstrated that the developmental upregulation of myelin-specific genes in mixed glial cultures is strongly attenuated by hypoglycemia. The present study was designed to evaluate the effect of hypoglycemia on differentiation-dependent upregulation of myelin genes in purified oligodendrocyte cultures. The expression of major myelin protein genes, i.e.,proteolipid protein (PLP), basic protein (BP) and myelin associated glycoprotein (MAG) were monitored by Northern blot analysis. In control cultures maintained at 6 mg/ml of glucose, the expression of all the genes upregulated rapidly, and plateaued at approximately day 4. A similar pattern of differentiation-dependent upregulation was observed for the gene encoding a lipogenic enzyme, i.e., malic enzyme(ME). In contrast to mixed glial cultures,however, this developmental gene upregulation was not significantly affected by severe hypoglycemia (approximately 0.02 mg/ml). The results indicate that the effect of glucose deprivation on oligodendrocyte genes observed inmixed glial cultures is mediated by other cells. The upregulation of the genes in differentiating oligodendrocytes was accompanied by the production of myelin-related membrane that was isolated by density gradient fractionation. In contrast to the effect on gene expression, this anabolic activity was highly dependent on glucose, as seen from a profound suppression by severe hypoglycemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:59:14