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Titolo:
Melatonin synthesis in the greenfrog retina in culture: I. Modulation by the light/dark cycle, forskolin and inhibitors of protein synthesis
Autore:
Alonso-Gomez, AL; Valenciano, AI; Alonso-Bedate, M; Delgado, MJ;
Indirizzi:
Univ Complutense, Fac Biol, Dpto Biol Anim 2, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 Biol Anim 2, E-28040 Madrid, Spain
Titolo Testata:
LIFE SCIENCES
fascicolo: 8, volume: 66, anno: 2000,
pagine: 675 - 685
SICI:
0024-3205(20000114)66:8<675:MSITGR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEROTONIN N-ACETYLTRANSFERASE; CHICKEN PINEAL-GLAND; CELL-CULTURES; PHOTORECEPTOR CELLS; CIRCADIAN CLOCK; CALCIUM INFLUX; JAPANESE-QUAIL; NAT ACTIVITY; LATERAL EYE; RANA-PEREZI;
Keywords:
melatonin; retina; frog; in vitro; NAT activity; light; retinal photoreceptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Alonso-Gomez, AL Univ Complutense, Fac Biol, Dept Biol Anim 2, Madrid 2840, Spain Univ Complutense Madrid Spain 2840 , Madrid 2840, Spain
Citazione:
A.L. Alonso-Gomez et al., "Melatonin synthesis in the greenfrog retina in culture: I. Modulation by the light/dark cycle, forskolin and inhibitors of protein synthesis", LIFE SCI, 66(8), 2000, pp. 675-685

Abstract

Melatonin is synthesized in the pineal gland and the retina of vertebrates. Retinal serotonin N-acetyltransferase (NAT) activity and melatonin show adaily rhythm with high levels during the dark phase of the photocycle. In some vertebrates, these retinal NAT and melatonin rhythms are maintained invitro. The aim of present work is to develop an eyecup culture system for the greenfrog (Rana perezi), suitable to analyze the mechanisms of regulation of melatonin synthesis by simultaneous determination of NAT activity andmelatonin release. The R. perezi eyecups released melatonin to the culturemedium in a rhythmic manner at least over a 27-h period under photocycle conditions. NAT activity and melatonin rhythms were similar to that observedin vivo under natural environmental conditions. Rana perezi retina exhibits a pronounced photosensitivity in vitro. Forskolin increased up to 2-fold the NAT activity and 4-fold the melatonin production at any lighting conditions. The addition of the translation inhibitor, cycloheximide, to the medium reduced significantly both nocturnal NAT activity and melatonin release,suggesting that de novo protein synthesis is produced daily during darkness. Actinomycin D, a transcription inhibitor, needs a longer time of action,because pre-existing mRNA must be depleted before the inhibition of melatonin release can be observed. The eyecup culture system is highly sensitive to light and chemical factors, which makes it particularly suitable as a model for the neurochemical analysis of melatonin biosynthesis in the retina of Rana perezi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 10:05:53