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Titolo:
Expression of the imprinted tumour-suppressor gene H19 is tightly regulated during normal haematopoiesis and is reduced in haematopoietic precursors of patients with the myeloproliferative disease polycythaemia vera
Autore:
Nunez, C; Bashein, AM; Brunet, CL; Hoyland, JA; Freemont, AJ; Buckle, AM; Murphy, C; Cross, MA; Lucas, G; Bostock, VJ; Brady, G;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England Univ Manchester, Dept Med, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England UMIST, Dept Biomol Sci, Leukaemia Res Fund, Cellular Dev Grp, Manchester M60 1QD, Lancs, England UMIST Manchester Lancs England M60 1QD Manchester M60 1QD, Lancs, England Univ Leipzig, IZKF, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 zig, IZKF, D-04103 Leipzig, Germany Manchester Royal Infirm, Dept Clin Haematol, Manchester M13 9WL, Lancs, England Manchester Royal Infirm Manchester Lancs England M13 9WL , Lancs, England
Titolo Testata:
JOURNAL OF PATHOLOGY
fascicolo: 1, volume: 190, anno: 2000,
pagine: 61 - 68
SICI:
0022-3417(200001)190:1<61:EOTITG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-II; POLYCYTHEMIA-VERA; WILMS-TUMOR; ERYTHROPOIETIN RECEPTOR; ERYTHROID PROGENITORS; MESSENGER-RNA; SINGLE CELLS; STEM-CELL; IN-VITRO; IGF2;
Keywords:
leukaemia; RT-PCR; tumour suppressor; H19; IGF2; lineage commitment;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Brady, G Univ Manchester, Sch Biol Sci, Stopford Bldgt G38,Oxford Rd, Manchester M13 9PT, Lancs, England Univ Manchester Stopford Bldgt G38,Oxford RdManchester Lancs England M13 9PT
Citazione:
C. Nunez et al., "Expression of the imprinted tumour-suppressor gene H19 is tightly regulated during normal haematopoiesis and is reduced in haematopoietic precursors of patients with the myeloproliferative disease polycythaemia vera", J PATHOLOGY, 190(1), 2000, pp. 61-68

Abstract

cDNA subtraction was employed to uncover differences in gene expression between myeloproliferative polycythaemia vera (PV) and normal haematopoietic precursors, Following cDNA subtraction using mRNAs isolated from PV and normal CD34+/CD33- bone-marrow cells, expression of the tumour suppressor H19 was found to be lowe or absent in the PV sample, Low levels of H19 expression in PV patients were confirmed by in situ hybridization, Using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to examine expression in the pluripotent haematopoietic cell Line FDCP-mix and single bone-marrow precursors, unambiguous IGF2 and H19 expression was demonstrated in normal haematopoietic precursors. Examination of individual bone-marrow precursors revealed that all IGF2-expressing haematopoietic precursors also co-expressed H19, indicating that H19 and IGF2 may be co-ordinately regulated during haematopoiesis. Analysis of FDCP-mix undergoing differentiation and single pluripotent and committed bone-marrow precursors revealed thatthe pattern of H19 expression coincided with the commitment to a single lineage. Taken together, these observations demonstrate that H19 and IGF2 arespecifically expressed during haematopoiesis and that low levels of H19 expression are associated with PV and may contribute to the pathology of the disease. Copyright (C) 2000 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 17:25:27