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Titolo:
Cyclic GMP/cyclic GMP-dependent protein kinase system prevents excitotoxicity in an immortalized oligodendroglial cell line
Autore:
Yoshioka, A; Yamaya, Y; Saiki, S; Kanemoto, M; Hirose, G; Pleasure, D;
Indirizzi:
Kanazawa Med Univ, Dept Neurol, Ishikawa 9200293, Japan Kanazawa Med UnivIshikawa Japan 9200293 Neurol, Ishikawa 9200293, Japan Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 2, volume: 74, anno: 2000,
pagine: 633 - 640
SICI:
0022-3042(200002)74:2<633:CGGPKS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; GLUTAMATE-RECEPTOR; RAT-BRAIN; MULTIPLE-SCLEROSIS; AMYLOID PRECURSOR; CALCIUM INFLUX; GLIAL-CELLS; EXPRESSION; DEATH; NMDA;
Keywords:
non-NMDA glutamate receptors; oligodendroglia; cyclic GMP; Ca2+ influx; cyclic GMP-dependent protein kinase; protein phosphatases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshioka, A Kanazawa Med Univ, Dept Neurol, 1-1 Daigaku, Ishikawa 9200293,Japan Kanazawa Med Univ 1-1 Daigaku Ishikawa Japan 9200293 3, Japan
Citazione:
A. Yoshioka et al., "Cyclic GMP/cyclic GMP-dependent protein kinase system prevents excitotoxicity in an immortalized oligodendroglial cell line", J NEUROCHEM, 74(2), 2000, pp. 633-640

Abstract

Previously, we have demonstrated that excitotoxicity of oligodendrocyte-like cells (OLC), differentiated from immortalized rat O-2A progenitor cells (CG-4 cells), is prevented by cyclic AMP-elevating agents. We now report that some agents that elevate cyclic GMP prevent OLC excitotoxicity. Kainate-induced injury was prevented by cyclic GMP analogues (8-bromo-cyclic GMP and dibutyryl cyclic GMP), a guanylate cyclase activator [atrial natriuretic peptide (ANP)], and phosphodiesterase inhibitors [3-isobutyl-1-methylxanthine (IBMX), ibudilast, propentofylline, and rolipram]. When both forskolin and 8-bromo-cyclic GMP were added, kainate-induced injury was additively prevented. There was a strong positive correlation between suppression of kainate-induced Ca2+ influx and prevention of injury by these chemicals. The measurement of intracellular cyclic AMP and cyclic GMP by radioimmunoassay demonstrated the following: an increase of cyclic GMP with treatment with 8-bromo-cyclic GMP, dibutyryl cyclic GMP, and ANP; an increase of cyclic AMP with treatment with ibudilast and rolipram; and an increase of both cyclic AMP and cyclic GMP with treatment with IBMX and propentofylline. Kainate-induced Ca2+ influx was decreased by 8-(4-chlorophenylthiol)-guanosine-3',5'-monophosphate, an activator of cyclic GMP-dependent protein kinase (PKG), orokadaic acid, an inhibitor of protein phosphatases 1 and 2A, RT-PCR and western blotting of OLC demonstrated transcription of PKG II gene and translation of PKG I beta mRNA, but no:translation of PKG I alpha mRNA, Therefore,we concluded that the cyclic GMP/PKG system prevents OLC excitotoxicity.

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Documento generato il 11/07/20 alle ore 19:44:18