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Titolo:
Efficient gene delivery into adult cardiomyocytes by recombinant Sindbis virus
Autore:
Datwyler, DA; Eppenberger, HM; Koller, D; Bailey, JE; Magyar, JP;
Indirizzi:
Swiss Fed Inst Technol, Inst Cell Biol, CH-8093 Zurich, Switzerland Swiss Fed Inst Technol Zurich Switzerland CH-8093 93 Zurich, Switzerland Cytos Biotechnol Inc, CH-8952 Zurich, Switzerland Cytos Biotechnol Inc Zurich Switzerland CH-8952 8952 Zurich, Switzerland Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland SwissFed Inst Technol Zurich Switzerland CH-8093 93 Zurich, Switzerland
Titolo Testata:
JOURNAL OF MOLECULAR MEDICINE-JMM
fascicolo: 12, volume: 77, anno: 1999,
pagine: 859 - 864
SICI:
0946-2716(199912)77:12<859:EGDIAC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEMLIKI-FOREST-VIRUS; CARDIAC MYOCYTES; CELLS; ADENOVIRUS; EXPRESSION; VECTORS; SYSTEM;
Keywords:
adult cardiomyocytes; Sindbis virus; gene transfer; heart; LacZ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Magyar, JP Swiss Fed Inst Technol, Inst Cell Biol, CH-8093 Zurich, Switzerland Swiss Fed Inst Technol Zurich Switzerland CH-8093 Switzerland
Citazione:
D.A. Datwyler et al., "Efficient gene delivery into adult cardiomyocytes by recombinant Sindbis virus", J MOL MED-J, 77(12), 1999, pp. 859-864

Abstract

Somatic gene therapy as a potential strategy for the treatment of myocardial diseases relies on an efficient gene transfer into cardiac muscle cells. The difficulty of delivering genes into adult cardiomyocytes exists not only in vivo but also in primary culture systems. Therefore, possibilities for ex vivo gene transfer and the in vitro study of physiological processes by reverse genetics are limited. We investigated the potential of an alphavirus-based vector system to transduce adult rat cardiomyocytes (ARC) in culture using a replication-deficient Sindbis virus (SIN) encoding beta-galactosidase (SIN-LacZ). Transduction efficiency depended on the virus concentration used, with expression of the reporter gene being detectable in up to 80% of cultured ARC as early as 24 h after infection. We observed a remarkably lower cytotoxicity of this viral vector in ARC than in other cells such as fibroblasts and neonatal cardiomyocytes. Additionally, no perceptible changes in the morphology of the nuclei or cytoskeleton were found in ARC 48 hafter infection with SIN-LacZ. We conclude that SIN vectors are useful forgene delivery into adult cardiomyocytes and believe that improved versionsof this viral system may be useful for cardiovascular gene therapy in the future.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 17:08:32