Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Competitive immunoassay for antigenic latex protein measurement: Rabbit antiserum-based assay compared to modified Lowry and human IgE-inhibition methods
Autore:
Chabane, H; Barbara, J; Hrabina, M; Leynadier, F;
Indirizzi:
Hop Rothschild AP HP, Ctr Allergie, F-75571 Paris, France Hop Rothschild AP HP Paris France F-75571 llergie, F-75571 Paris, France
Titolo Testata:
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY & CLINICAL IMMUNOLOGY
fascicolo: 6, volume: 9, anno: 1999,
pagine: 372 - 379
SICI:
1018-9068(199911/12)9:6<372:CIFALP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-RUBBER LATEX; SKIN PRICK TEST; ALLERGEN; EXTRACTS;
Keywords:
latex proteins; gloves; allergy; ELISA; allergens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Chabane, H Univ Paris, Lab Rech Immuno Allergol, 33 Blvd Picpus, F-75571 Paris 12, France Univ Paris 33 Blvd Picpus Paris France 12 571 Paris 12, France
Citazione:
H. Chabane et al., "Competitive immunoassay for antigenic latex protein measurement: Rabbit antiserum-based assay compared to modified Lowry and human IgE-inhibition methods", J INVES ALL, 9(6), 1999, pp. 372-379

Abstract

A new rabbit antiserum-based assay was designed and evaluated for the assessment of the allergen potency of latex medical gloves, since total proteinmeasurement by modified Lowry method remains unsatisfactory and the human IgE-inhibition method is limited by the use of sera from type I allergic patients. Four rabbit sera against a nonammoniated latex extract were shown by immunoblotting to have similar binding patterns to those obtained by human IgE. One rabbit serum was used to develop a competitive immunoassay for antigenic latex proteins (CIALP). The same nonammoniated latex extract was used for coating and calibration. The lower detection limit of the method was 0.085 mu g/g of glove. Antigenic proteins measured by CIALP for 77 latex glove extracts (33 pairs of surgical gloves and II exam gloves) showed positive correlation with those of the modified Lowry method (r = 0.4; p <0.001). For 36 extracts made from the right and the left hand of 18 out of the 33 surgical latex gloves tested inhibition of human specific-IgE results didnot correlate with the modified Lowry method but did with the CIALP results (r = 0.8, p <0.0001). The CIALP, which is well correlated with the human IgE-inhibition test, enables the assessment of the allergenicity of latex medical gloves by measuring the antigenic proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:47:33