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Titolo:
Effects of liver-derived dendritic cell progenitors on Th1-and Th2-like cytokine responses in vitro and in vivo
Autore:
Khanna, A; Morelli, AE; Zhong, CP; Takayama, T; Lu, L; Thomson, AW;
Indirizzi:
Univ Pittsburgh, Med Ctr, Dept Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 pt Surg, Pittsburgh, PA 15213 USA Univ Pittsburgh, Thomas E Starzl Transplantat Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 at Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Biochem, Pittsburgh, PA 15213 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 3, volume: 164, anno: 2000,
pagine: 1346 - 1354
SICI:
0022-1767(20000201)164:3<1346:EOLDCP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CD4(+) T-CELLS; ANTIGEN-PRESENTING FUNCTION; MOUSE BONE-MARROW; ALLOGRAFT-REJECTION; LYMPHOID-TISSUES; IN-VIVO; MONOCLONAL-ANTIBODY; MIGRATION PATTERNS; HEART ALLOGRAFTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, L Univ Pittsburgh, Med Ctr, Dept Surg, W1540,Biomed Sci Tower,200 Lothrop St, Pittsburgh, PA 15213 USA Univ Pittsburgh W1540,Biomed Sci Tower,200Lothrop St Pittsburgh PA USA 15213
Citazione:
A. Khanna et al., "Effects of liver-derived dendritic cell progenitors on Th1-and Th2-like cytokine responses in vitro and in vivo", J IMMUNOL, 164(3), 2000, pp. 1346-1354

Abstract

There is evidence that donor-derived dendritic cells (DC), particularly those at a precursor/immature stage, may play a role in the immune privilege of liver allografts, Underlying mechanisms are poorly understood. We have examined the influence of in vitro generated mouse liver-derived DC progenitors (DCp) on proliferative, cytotoxic, and Th1/Th2 cytokine responses induced in allogeneic T cells. Liver DCp, propagated in GM-CSF from C57B10 mice (H2(b)), induced only minimal proliferation, and weak cytotoxic responses in allogeneic (C3H; H2(k)) T cells compared with mature bone marrow (BM)-derived DC. Flow-cytometric analysis of intracellular cytokine staining revealed that mature BM DC but not liver DCp, elicited CD4(+) T cell production of IFN-gamma. Intracellular expression of IL-10 was very low in both BM DC- and liver DCp-stimulated CD4(+) T cells. Only stimulation by liver DCp was associated with IL-10 secretion in primary MLR, Notably, these liver DCp cocultured with allogeneic T cells stained strongly for IL-IO, Following local (s,c,) injection in allogeneic recipients, both BM DC and liver DCp homedto T cell areas of draining lymph nodes and spleen, where they were readily detected by immunohistochemistry up to 2 wk postinjection, Liver DCp induced clusters of IL-10- and IL-4-secreting mononuclear cells, whereas Th2 cytokine-secreting cells were not detected in mice injected with mature BM DC. By contrast, comparatively high numbers of IFN-gamma(+) cells were induced by BM DC, Modulation of Th2 cytokine production by donor-derived DCp may contribute to the comparative immune privilege of hepatic allografts.

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Documento generato il 14/07/20 alle ore 05:23:24