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Titolo:
Cold preservation of fatty liver grafts: prevention of functional and ultrastructural impairments by venous oxygen persufflation
Autore:
Minor, T; Akbar, S; Tolba, R; Dombrowski, F;
Indirizzi:
Univ Bonn, Surg Clin, Div Surg Res, D-53127 Bonn, Germany Univ Bonn BonnGermany D-53127 Clin, Div Surg Res, D-53127 Bonn, Germany Univ Bonn, Inst Pathol, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300 iv Bonn, Inst Pathol, D-5300 Bonn, Germany
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 1, volume: 32, anno: 2000,
pagine: 105 - 111
SICI:
0168-8278(200001)32:1<105:CPOFLG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
KUPFFER CELL ACTIVATION; HEART-BEATING DONORS; WARM ISCHEMIC INSULT; RAT-LIVER; REPERFUSION INJURY; ORGAN PRESERVATION; GASEOUS OXYGEN; TRANSPLANTATION; STEATOSIS;
Keywords:
fatty liver; HTK; ischemia; marginal donor; mitochondria; oxygen persufflation; preservation; steatosis; transplantation; VSOP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Minor, T Univ Bonn, Surg Clin, Div Surg Res, Siegmund Freud Str 25, D-53127 Bonn, Germany Univ Bonn Siegmund Freud Str 25 Bonn Germany D-53127 nn, Germany
Citazione:
T. Minor et al., "Cold preservation of fatty liver grafts: prevention of functional and ultrastructural impairments by venous oxygen persufflation", J HEPATOL, 32(1), 2000, pp. 105-111

Abstract

Background/Aims: The incidence of steatosis in livers retrieved for organ transplantation is up to 30%, Due to the shortage of donor organs, many of these livers are accepted for clinical transplantation, although a high rate of graft dysfunction is associated with ischemic preservation of steatotic livers. The present study was intended to reduce the ischemia/reperfusioninjury of steatotic grafts by the use of venous systemic oxygen persufflation during cold storage,Methods: A histologically-documented mild to moderate steatosis was induced in livers of Wistar rats by fasting for 2 days and subsequent feeding of a fat-free diet enriched in carbohydrates. Fatty livers were retrieved and flushed via the portal vein with 60 mi of HTK. In group A, livers were thenstored ischemically at 4 degrees C for 24 h, Livers of group B were additionally connected to a gaseous oxygen supply and persufflated with O-2 via the venous vascular system during the cold storage period, Viability of the livers was then assessed upon isolated perfusion in vitro with oxygenated Krebs-Henseleit buffer,Results: Venous systemic oxygen sufflation resulted in a relevant and significant reduction of parenchymal (ALT: 132+/-90 vs 434+/-172 U/l; p<0.01) and mitochondrial (GLDH: 116+/-57 vs 633+/-241 U/l; p<0.001) enzyme release during reperfusion. Moreover, Kupffer cell activation, as evaluated from acid phosphatase activity in the perfusate, was reduced to about 1/3 (4.0+/-1.3 vs 11.9+/-5.3 U/l; p<0.01). Electron microscopic analysis revealed that the liver mitochondria and sinusoidal endothelial lining were better preserved after oxygen persufflation, which was in line with the data on enzyme release and the increased portal perfusion pressure in the untreated group, while normal values were found after venous systemic oxygen sufflation,Conclusion: Venous oxygen persufflation may thus represent a useful tool for the safe and improved preservation of ischemia-sensitive steatotic livers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:31:12