Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Purification of a D-hydantoinase using a laboratory-scale Streamline phenyl column as the initial step
Autore:
Abendroth, J; Chatterjee, S; Schomburg, D;
Indirizzi:
Univ Cologne, Inst Biochem, D-50674 Cologne, Germany Univ Cologne Cologne Germany D-50674 t Biochem, D-50674 Cologne, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 737, anno: 2000,
pagine: 187 - 194
SICI:
1387-2273(20000114)737:1-2<187:POADUA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
THERMOSTABLE D-HYDANTOINASE;
Keywords:
purification; enzymes; D-hydantoinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Schomburg, D Univ Cologne, Inst Biochem, Zulpicher Str 47, D-50674 Cologne, Germany Univ Cologne Zulpicher Str 47 Cologne Germany D-50674 ermany
Citazione:
J. Abendroth et al., "Purification of a D-hydantoinase using a laboratory-scale Streamline phenyl column as the initial step", J CHROMAT B, 737(1-2), 2000, pp. 187-194

Abstract

A D-hydantoinase from Thermus sp. was overexpressed in Escherichia coli and purified to homogeneity for subsequent crystallization. The purification was performed with hydrophobic interaction chromatography as the capture step followed by anion-exchange chromatography and gel permeation chromatography as intermediate purification and polishing steps, respectively. The hydrophobic interaction step was done in fluidized bed mode in a laboratory-scale Streamline column made from conventional laboratory equipment. The whole purification protocol could be finished within one day. The purified enzyme crystallizes. The crystals are suitable for X-ray protein structure analysis and diffract to at least 2.3 Angstrom resolution. Complete data sets have been measured up to 2.6 Angstrom resolution. The X-ray structure is currently being solved. (C) 2000 Published by Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:43:51