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Titolo:
Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats
Autore:
Suzuki, S; Tanaka, K; Nogawa, S; Nagata, E; Ito, D; Dembo, T; Fukuuchi, Y;
Indirizzi:
Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Neurol, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
fascicolo: 11, volume: 19, anno: 1999,
pagine: 1256 - 1262
SICI:
0271-678X(199911)19:11<1256:TPACLO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; NECROSIS-FACTOR-ALPHA; ARTERY OCCLUSION; CHOLINERGIC NEURONS; ACTIVATED MICROGLIA; TNF-ALPHA; BRAIN; EXPRESSION; IL-6; ASTROCYTES;
Keywords:
focal ischemia; immunohistochemistry; interleukin-6; microglia; neuron; rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, S Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 yo 1608582, Japan
Citazione:
S. Suzuki et al., "Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats", J CEREBR B, 19(11), 1999, pp. 1256-1262

Abstract

Although interleukin-6 (IL-6) has various neuroprotective effects against cerebral ischemia, the topographic distribution and cellular source of IL-6after cerebral ischemia remain unclear. In the current study, the localization of IL-6 protein was immunohistochemically examined in rats after 3.5, 12, 24, and 48 hours of reperfusion after 1.5 hours of middle cerebral artery occlusion. Middle cerebral artery occlusion was induced by the intraluminal suture method. The specificity of the anti-IL-6 antibody used in the current study was confirmed by Western blot analysis and an immunoabsorption test. To identify the cellular source, lectin histochemical study and immunohistochemical study with microtubule-associated protein-2, ED1, and glial fibrillary acidic protein also were carried out. The sham group did not show any clear IL-6 immunoreactivity. After 3.5 hours of reperfusion, IL-6 inmunoreactivity was first detected on the reperfused side, and it was upregulated, especially in the periinfarct region, after 3.5 hours of reperfusion. Also, IL-6 was expressed after 3.5 hours of reperfusion in the contralateral cerebral cortex and bilateral hippocampi. Double staining showed that the cells containing IL-G were neurons and round-type microglia, not astrocytes. The current findings suggest that IL-6 expression in ischemically threatened neurons and reactive microglia is closely associated with brain tissue neuroprotective mechanisms against cerebral ischemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:30:01