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Titolo:
Anti-human immunodeficiency virus type 1 of a two-strand-system targeted to the polypurine tract
Autore:
Hiratou, T; Miyano-Kurosaki, N; Tsukahara, S; Tamura, Y; Takai, K; Yamamoto, N; Takaku, H;
Indirizzi:
Chiba Inst Technol, Dept Ind Chem, Narashino, Chiba 2750016, Japan Chiba Inst Technol Narashino Chiba Japan 2750016 no, Chiba 2750016, Japan Chiba Inst Technol, High Technol Res Ctr, Narashino, Chiba 2750016, Japan Chiba Inst Technol Narashino Chiba Japan 2750016 no, Chiba 2750016, Japan Tokyo Med & Dent Univ, Sch Med, Dept Microbiol, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 2, volume: 5, anno: 2000,
pagine: 125 - 132
SICI:
1107-3756(200002)5:2<125:AIVT1O>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIPLE-HELIX FORMATION; DEPENDENT DNA POLYMERASE; DEFICIENCY SYNDROME AIDS; FORMING OLIGONUCLEOTIDES; REVERSE-TRANSCRIPTASE; VIRAL-RNA; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; HIV-1 GP120; INHIBITION; BINDING;
Keywords:
triplex; two-strand-system; inhibition of HIV-1 replication; polypurine tract; MOLT-4 cells; MT-4 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Takaku, H Chiba Inst Technol, Dept Ind Chem, 2-17-1 Tsundanuma, Narashino,Chiba 2750016, Japan Chiba Inst Technol 2-17-1 Tsundanuma Narashino Chiba Japan 2750016
Citazione:
T. Hiratou et al., "Anti-human immunodeficiency virus type 1 of a two-strand-system targeted to the polypurine tract", INT J MOL M, 5(2), 2000, pp. 125-132

Abstract

The polypurine tract (PPT) is highly conserved among the known human immunodeficiency virus (HIV)-1 strains and is a possible target fnr triplex formation WP show the effects of triple-helix formation by assays of primer extension inhibition in vibro, using a two-strand-system (FTFOs) targeted to the PPT of HIV-1. The two-stranded composition of a triple-helix is thermodynamically and kinetically superior to the three-strand-system The FTFOs inhibited the RT activity in a sequence-specific manner. i.e., the tripler actually formed at the PPT and blocked the RT. The FTFOs containing the phosphorothioate groups at the antisense sequences showed greater 3'-exonuclease resistance. In the observation of the FITC-DsDGloopT5-37 with MOLT-4 cells by a confocal laser scanning microscope, diffuse fluorescence was apparently observed in the cytoplasm and nucleus. However, weak fluorescence was observed within the cytoplasm and nucleus of MOLT-4 cells treated with the antisense phosphorothioate oligonucleotides (S-ODN-gag-AUG). In HIV-1 infectedMOLT-4 cells, the FTFOs containing the phosphorothioate groups at the antisense sequence sites and guanosine rich parts within the third Hoogsteen base pairing sequence inhibit the replication of HIV-1 more effectively than the antisense oligonucleotides, indicating sequence-specific inhibition of HIV-1 replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:58:17