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Titolo:
Construction and characterization of an effector strain of Streptococcus mutans for replacement therapy of dental caries
Autore:
Hillman, JD; Brooks, TA; Michalek, SM; Harmon, CC; Snoep, JL; Van Der Weijden, CC;
Indirizzi:
Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 ral Biol, Gainesville, FL 32610 USA Univ Alabama, Dept Microbiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Microbiol, Birmingham, AL 35294 USA Free Univ Amsterdam, Dept Microbial Physiol, NL-1081 HV Amsterdam, Netherlands Free Univ Amsterdam Amsterdam Netherlands NL-1081 HV terdam, Netherlands
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 2, volume: 68, anno: 2000,
pagine: 543 - 549
SICI:
0019-9567(200002)68:2<543:CACOAE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-(+)-LACTATE DEHYDROGENASE-DEFICIENCY; HUMAN ORAL CAVITY; ALCOHOL-DEHYDROGENASE; ZYMOMONAS-MOBILIS; IMPLANTATION; LETHAL; COLONIZATION; BACTERIOCIN; EXPRESSION; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hillman, JD Univ Florida, Coll Dent, Dept Oral Biol, Box 100424, Gainesville, FL 32610USA Univ Florida Box 100424 Gainesville FL USA 32610 , FL 32610USA
Citazione:
J.D. Hillman et al., "Construction and characterization of an effector strain of Streptococcus mutans for replacement therapy of dental caries", INFEC IMMUN, 68(2), 2000, pp. 543-549

Abstract

An effector strain has been constructed for use in the replacement therapyof dental caries. Recombinant DNA methods were used to make the Streptococcus mutans supercolonizing strain, JH1140, lactate dehydrogenase deficient by deleting virtually all of the ldh open reading frame (ORF), To compensate for the resulting metabolic imbalance, a supplemental alcohol dehydrogenase activity was introduced by substituting the adhB ORF from Zymomonas mobilis in place of the deleted ldh ORF, The resulting clone, BCS3-L1, was found to produce no detectable lactic acid during growth on a variety of carbonsources, and it produced significantly Less total acid due to its increased production of ethanol and acetoin, BCS3-L1 was significantly less cariogenic than JH1140 in both gnotobiotic- and conventional-rodent models. It colonized the teeth of conventional rats as well as JH1140 in both aggressive-displacement and preemptive-colonization models. No gross or microscopic abnormalities of major organs were associated with oral colonization of rats with BCS3-L1 for 6 months. Acid-producing revertants of BCS3-L1 were not observed in samples taken from infected animals (reversion frequency, <10(-3)) or by screening cultures grown in vitro, where no revertants were observed among 10(5) colonies examined on pH indicator medium. The reduced pathogenic potential of BCS3-L1, its strong colonization potential, and its genetic stability suggest that this strain is well suited to serve as an effectorstrain in the replacement therapy of dental caries in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 06:22:35