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Titolo:
Dynein- and microtubule-mediated translocation of adenovirus serotype 5 occurs after endosomal lysis
Autore:
Leopold, PL; Kreitzer, G; Miyazawa, N; Rempel, S; Pfister, KK; Rodriguez-Boulan, E; Crystal, RG;
Indirizzi:
Cornell Univ, Weill Med Coll, Dept Ophthalmol, Dyson Vis Res Inst, New York, NY 10021 USA Cornell Univ New York NY USA 10021 n Vis Res Inst, New York, NY 10021 USA Cornell Univ, Weill Med Coll, New York Presbyterian Hosp, Div Pulm & Crit Care Med, New York, NY 10021 USA Cornell Univ New York NY USA 10021 Crit Care Med, New York, NY 10021 USA Univ Virginia, Hlth Sci Ctr, Dept Cell Biol, Charlottesville, VA 22908 USAUniv Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 1, volume: 11, anno: 2000,
pagine: 151 - 165
SICI:
1043-0342(20000101)11:1<151:DAMTOA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERPES-SIMPLEX VIRUS; FAST AXONAL-TRANSPORT; NUCLEAR-PORE COMPLEX; PENTON BASE PROTEIN; HELA-CELLS; GENE-TRANSFER; IN-VIVO; CYTOPLASMIC DYNEIN; EARLY EVENTS; DEPENDENT RELEASE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
102
Recensione:
Indirizzi per estratti:
Indirizzo: Crystal, RG Cornell Univ, Weill Med Coll, Dept Ophthalmol, Dyson Vis Res Inst, 520 E 70th St,ST 505, New York, NY 10021 USA Cornell Univ 520 E 70th St,ST 505 New York NY USA 10021 21 USA
Citazione:
P.L. Leopold et al., "Dynein- and microtubule-mediated translocation of adenovirus serotype 5 occurs after endosomal lysis", HUM GENE TH, 11(1), 2000, pp. 151-165

Abstract

Modified viruses are used as gene transfer vectors because of their ability to transfer genetic material efficiently to the nucleus of a target cell,To better understand intracellular translocation of adenovirus serotype 5 (Ad), fluorophores were covalently conjugated to Ad capsids, and movement of fluorescent Ad within the cytoplasm was observed during the first hour ofinfection of a human lung epithelial carcinoma cell line (A549). Ad translocation was characterized with respect to its ability to achieve nuclear envelope localization as well as directed movement in the cytoplasm, Whereas Ad achieved efficient nuclear localization 60 min after infection of A549 cells under control conditions, depolymerization of the microtubule cytoskeleton by addition of 25 mu M nocodazole reversibly inhibited development of nuclear localization, In contrast, depolymerization of microfilaments by addition of 1 mu M cytochalasin D had no effect on nuclear localization, Direct video observation of Ad motility showed that nocodazole, but not cytochalasin D, caused a reversible decrease in rapid linear translocations of Ad in the cytoplasm of A549 cells, Microinjection of function-blocking antibodies against the microtubule-dependent motor protein, cytoplasmic dynein, but not kinesin, blocked nuclear localization of Ad, consistent with net minus end-directed motility indicated by accumulation of Ad at mitotic spindles, Fluorescence ratio imaging revealed a neutral pH in the environment of translocating Ad, leading to a model in which the interaction of Ad with an intact microtubule cytoskeleton and functional cytoplasmic dynein occurs after escape from endosomes and is a necessary prerequisite to nuclear localization of adenovirus serotype 5.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:52:17