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Titolo:
Genetically modified CD34(+) cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model
Autore:
Gomez-Navarro, J; Contreras, JL; Arafat, W; Jiang, XL; Krisky, D; Oligino, T; Marconi, P; Hubbard, B; Glorioso, JC; Curiel, DT; Thomas, JM;
Indirizzi:
Transplant Immunobiol, Dept Surg, Birmingham, AL 35294 USA Transplant Immunobiol Birmingham AL USA 35294 g, Birmingham, AL 35294 USA Gene Therapy Ctr, Birmingham, AL USA Gene Therapy Ctr Birmingham AL USAGene Therapy Ctr, Birmingham, AL USA Dept Mol Genet & Biochem, Pittsburgh, PA 15261 USA Dept Mol Genet & Biochem Pittsburgh PA USA 15261 Pittsburgh, PA 15261 USA
Titolo Testata:
GENE THERAPY
fascicolo: 1, volume: 7, anno: 2000,
pagine: 43 - 52
SICI:
0969-7128(200001)7:1<43:GMCCAC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; ENDOTHELIAL-CELLS; ADENOVIRUS VECTORS; PROGENITOR CELLS; PRECURSOR CELLS; STEM-CELLS; THERAPY; TRANSDUCTION; EFFICIENCY; EXPRESSION;
Keywords:
rhesus monkey; CD34 cell; gene transfer; herpes simplex virus; herpes simplex virus thymidine kinase; anti-angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Thomas, JM Transplant Immunobiol, Dept Surg, 1808 7th Ave S,BDB 563, Birmingham, AL 35294 USA Transplant Immunobiol 1808 7th Ave S,BDB 563 BirminghamAL USA 35294
Citazione:
J. Gomez-Navarro et al., "Genetically modified CD34(+) cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model", GENE THER, 7(1), 2000, pp. 43-52

Abstract

To develop a cellular vehicle able to reach systemically disseminated areas of angiogenesis, we sought to exploit the natural tropism of circulating endothelial progenitor cells (EPCs). Primate CD34(+) EPCs were genetically modified with high efficiency and minimal toxicity using a non-replicative herpes virus vector These EPCs localized in a skin autograft model of angiogenesis in rhesus monkeys, and sustained the expression of a reporter gene for several weeks while circulating in the blood. In animals infused with autologous CD34(+) EPCs transduced with a thymidine kinase-encoding herpes virus, skin autografts and subcutaneous Matrigel pel lets impregnated with vascular growth factors underwent necrosis or accelerated regression after administration of ganciclovir. Importantly, the whole intervention was perfectly well tolerated. The accessibility, easy manipulation, lack of immunogenicity of the autologous CD34+ cell vehicles, and tropism for areas of angiogenesis render autologous CD34+ circulating endothelial progenitors as ideal candidates for exploration of their use as cellular vehicles when systemic gene delivery to those areas is required.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 20:19:39