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Titolo:
Histamine depletion does not affect pancreastatin secretion from isolated rat stomach ECL cells
Autore:
Lindstrom, E; Norlen, P; Hakanson, R;
Indirizzi:
Univ Lund, Dept Pharmacol, Inst Physiol Sci, S-22362 Lund, Sweden Univ Lund Lund Sweden S-22362 ol, Inst Physiol Sci, S-22362 Lund, Sweden
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 1, volume: 387, anno: 2000,
pagine: 19 - 25
SICI:
0014-2999(20000103)387:1<19:HDDNAP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROCHROMAFFIN-LIKE CELLS; VESICULAR MONOAMINE TRANSPORTER; GASTRIC-ACID SECRETION; ALPHA-FLUOROMETHYLHISTIDINE; CHROMAFFIN GRANULE; AMINE TRANSPORTER; RESERPINE BINDING; ENDOCRINE-CELLS; EXPRESSION; INHIBITORS;
Keywords:
bafilomycin A(1); alpha-fluoromethylhistidine; histidine decarboxylase; reserpine; vacuolar-type ATPase; vesicular monoamine transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Hakanson, R Univ Lund, Dept Pharmacol, Inst Physiol Sci, Solvegatan 10, S-22362 Lund, Sweden Univ Lund Solvegatan 10 Lund Sweden S-22362 2362 Lund, Sweden
Citazione:
E. Lindstrom et al., "Histamine depletion does not affect pancreastatin secretion from isolated rat stomach ECL cells", EUR J PHARM, 387(1), 2000, pp. 19-25

Abstract

ECL cells co-secrete histamine and pancreastatin, a chromogranin A-derivedpeptide, in response to gastrin. The aim of the study was to explore possible ways to deplete ECL cells of histamine without affecting pancreastatin and to examine how histamine depletion affects pancreastatin secretion. Isolated rat stomach ECL cells (80-85% purity), prepared by counter-flow elutriation, were cultured for 48 h in the presence of alpha-fluoromethylhistidine (histidine decarboxylase inhibitor), bafilomycin A(1) (inhibitor of vacuolar-type proton-translocating ATPase or reserpine (inhibitor of vesicular monoamine transporter). At this stage, the cells were challenged with 10 nM(EC100) gastrin-17 for 30 min. Histamine and pancreastatin were determinedby radioimmunoassay. Maximally effective concentrations of alpha-fluoromethylhistidine, bafilomycin A(1) and reserpine were found to lower ECL-cell histamine (by 60%, 78% and 80%, respectively) without affecting pancreastatin. Basal histamine secretion was reduced in a dose-dependent manner by all three drugs. Gastrin-evoked histamine secretion was reduced greatly by the three agents, while pancreastatin secretion was unaffected. The results show that histamine can be depleted not only by inhibiting its formation (a-fluoromethylhistidine), but also land more effectively) by inhibiting histamine vesicular uptake, directly (reserpine) or indirectly (bafilomycin A,). The results also indicate that although histamine is co-stored with pancreastatin, it is not required for either storage or secretion of pancreastatin. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:40:29