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Titolo:
Involvement of insulin-like factor 3 (Insl3) in diethylstilbestrol-inducedcryptorchidism
Autore:
Emmen, JMA; Mcluskey, A; Adham, IM; Engel, W; Verhoef-Post, M; Themmen, APN; Grootegoed, JA; Brinkmann, AO;
Indirizzi:
Erasmus Univ, Dept Endocrinol & Reprod, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands Univ Gottingen, Inst Human Genet, D-37073 Gottingen, Germany Univ Gottingen Gottingen Germany D-37073 net, D-37073 Gottingen, Germany
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 2, volume: 141, anno: 2000,
pagine: 846 - 849
SICI:
0013-7227(200002)141:2<846:IOIF3(>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROIDOGENIC FACTOR-I; FETAL-RAT TESTIS; TESTICULAR DESCENT; HORMONAL-CONTROL; MULLERIAN DUCT; MICE; EXPRESSION; ESTROGEN; MOUSE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Emmen, JMA Erasmus Univ, Dept Endocrinol & Reprod, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam Netherlands NL-3000 DR erlands
Citazione:
J.M.A. Emmen et al., "Involvement of insulin-like factor 3 (Insl3) in diethylstilbestrol-inducedcryptorchidism", ENDOCRINOL, 141(2), 2000, pp. 846-849

Abstract

Recently, it has been shown that targeted inactivation of the Insl3 gene in male mice results in cryptorchidism. The Insl3 gene encodes insulin-like factor 3 (lnsl3), which is expressed in fetal Leydig cells. The testicular factor Insl3 appears to play an important role in the transabdominal phase of testis descent, which involves development of the gubernaculum. Other studies have demonstrated that in utero exposure to diethylstilbestrol (DES),a synthetic estrogen, can lead to cryptorchidism both in humans and in animal models. The present study was undertaken to investigate whether prenatal DES-exposure might interfere with testicular Insl3 mRNA expression. Furthermore, theeffect of DES on steroidogenic factor 1 (SF-1) mRNA expression level was determined, since it has been shown that SF-1 plays an essential role in transcriptional activation of the Insl3 gene promoter. Timed pregnant mice were treated with DES (100 mu g/kg body weight) or vehicle alone on days E9 (gestational day 9) through E17. Control and DES-exposed mouse fetuses were collected at E16, E17 and E18, when transabdominal testis descent is taking place. Lack of gubernaculum development in DES-exposed animals was confirmed by histological analyses at E17. Expression of Insl3 and SF-1 mRNAs was studied in testes of control and DES-exposed fetuses at E16 and E18 by RNaseprotection assay. Prenatal DES-exposure resulted in a three-fold decrease in Insl3 mRNA expression level (P < 0.005), at both E16 and E18. In contrast, DES treatment had no effect on the expression of SF-1 mRNA. These results support our hypothesis that DES may interfere with gubernaculum development by altering Insl3 mRNA expression, providing a possible mechanism by which DES may cause cryptorchidism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:54:32