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Titolo:
Stage-specific expression of pituitary adenylate cyclase-activating polypeptide type I receptor messenger ribonucleic acid during ovarian follicle development in the rat
Autore:
Park, KJ; Lee, J; Wang, L; Park, JH; Kwon, HB; Arimura, A; Chun, SY;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Tulane Univ, Hebert Ctr, US Japan Biomed Res Labs, Belle Chasse, LA 70037 USA Tulane Univ Belle Chasse LA USA 70037 es Labs, Belle Chasse, LA 70037 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 2, volume: 141, anno: 2000,
pagine: 702 - 709
SICI:
0013-7227(200002)141:2<702:SEOPAC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE INTESTINAL PEPTIDE; GRANULOSA-CELLS; STIMULATING-HORMONE; FUNCTIONAL EXPRESSION; HYPOTHALAMIC PEPTIDE; PREOVULATORY FOLLICLES; LUTEINIZING-HORMONE; TISSUE DISTRIBUTION; MOLECULAR-CLONING; PACAP RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Chun, SY Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
K.J. Park et al., "Stage-specific expression of pituitary adenylate cyclase-activating polypeptide type I receptor messenger ribonucleic acid during ovarian follicle development in the rat", ENDOCRINOL, 141(2), 2000, pp. 702-709

Abstract

Expression of pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide with considerable homology to vasoactive intestinal peptide,has been shown to be stimulated by gonadotropins in the ovary. The presentstudies further evaluated the cell-type specific expression and gonadotropin regulation of PACAP type I receptor (PACAPR) messenger RNA in immature rat ovaries and in cultured preovulatory follicles. Northern blot analysis of ovaries obtained from prepubertal rats revealed the increased expression of PACAPR during prepubertal development. The major cell types expressing PACAPR messenger RNA were granulosa cells of large preantral follicles. Treatment of immature rats with PMSG caused a decrease in ovarian PACAPR expression. In contrast, treatment with human (h) CG at 2 days after PMSG treatment stimulated ovarian PACAPR messenger RNA within 3-6 h in granulosa cells of preovulatory follicles. Treatment of cultured preovulatory follicles in vitro with LH further confirmed the time- and dose-dependent stimulation ofPACAPR by gonadotropins in granulosa cells of preovulatory follicles. Moreover, RNase protection assay revealed that the short variant of ovarian PACAPR was the predominant form stimulated during prepubertal development and by gonadotropins. These results demonstrate the expression of PACAPR messenger RNA in granulosa cells of growing follicles and of preovulatory follicles stimulated by gonadotropins, and suggest that PACAP may play a role in the growth of developing follicles and in ovulation as an autocrine/paracrine factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 01:00:13