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Titolo:
Assays of ligand-human serum albumin binding using pulsed ultrafiltration and liquid chromatography-mass spectrometry
Autore:
Gu, CG; Nikolic, D; Lai, J; Xu, XY; van Breemen, RB;
Indirizzi:
Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 & Pharmacognosy, Chicago, IL 60612 USA
Titolo Testata:
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
fascicolo: 6, volume: 2, anno: 1999,
pagine: 353 - 359
SICI:
1386-2073(199912)2:6<353:AOLSAB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMBINATORIAL LIBRARIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: van Breemen, RB Univ Illinois, Dept Med Chem & Pharmacognosy, 833 S Wood St, Chicago, IL 60612 USA Univ Illinois 833 S Wood St Chicago IL USA 60612 60612 USA
Citazione:
C.G. Gu et al., "Assays of ligand-human serum albumin binding using pulsed ultrafiltration and liquid chromatography-mass spectrometry", COMB CHEM H, 2(6), 1999, pp. 353-359

Abstract

Two approaches were utilized to increase the throughput of pulsed ultrafiltration assays of ligand binding to human serum albumin, reducing the volume of the ultrafiltration chamber and combining pulsed ultrafiltration with high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-MS). Affinity constants for binding of ligands to human serum albumin were determined using pulsed ultrafiltration with ultraviolet absorbance detection. The first affinity constants (K-a1) were measured for the binding of dansylsarcosine, dansylamide, 7-anilinocoumarin-4-acetic acid and warfarin, and were determined to be 1.8 x 10(5), 5 x 10(4),8 x 10(4,) and 2.0 x 105 M-1, respectively. The throughput of pulsed ultrafiltration analyses was tripled compared to previous pulsed ultrafiltrationmeasurements by reducing the volume of the chamber. In addition, the use of LC-MS with pulsed ultrafiltration permitted the simultaneous comparison and rank ordering of ligand mixtures for binding to serum albumin. For example, the throughput of these pulsed ultrafiltration measurements was tripledby analyzing three ligands as a mixture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:17:54