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Titolo:
Oxidized low-density lipoproteins inhibit endothelial cell proliferation by suppressing basic fibroblast growth factor expression
Autore:
Chen, CH; Jiang, W; Via, DP; Luo, S; Li, TR; Lee, YT; Henry, PD;
Indirizzi:
Baylor Coll Med, Dept Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Med, Dept Med, Houston, TX 77030 USA Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 100, Taiwan Natl Taiwan Univ Hosp Taipei Taiwan 100 Internal Med, Taipei 100, Taiwan
Titolo Testata:
CIRCULATION
fascicolo: 2, volume: 101, anno: 2000,
pagine: 171 - 177
SICI:
0009-7322(20000118)101:2<171:OLLIEC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-ACTIVATING-FACTOR; SMOOTH-MUSCLE CELLS; MESSENGER-RNA; IN-VIVO; ANGIOGENESIS; LDL; RABBIT; HYPERCHOLESTEROLEMIA; LIPOPOLYSACCHARIDE; PHOSPHOLIPIDS;
Keywords:
lipoproteins; growth substances; endothelium; genes; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, CH Baylor Coll Med, Dept Med, 6565 Fannin,MS A-601, Houston, TX 77030 USA Baylor Coll Med 6565 Fannin,MS A-601 Houston TX USA 77030 030 USA
Citazione:
C.H. Chen et al., "Oxidized low-density lipoproteins inhibit endothelial cell proliferation by suppressing basic fibroblast growth factor expression", CIRCULATION, 101(2), 2000, pp. 171-177

Abstract

Background-Hyperlipidemia inhibits proliferation of endothelial cells (ECs) in culture and angiogenesis in vivo and in arterial explants. Elucidationof the mechanisms may suggest novel therapies against atherosclerosis. Methods and Results-Basic Fibroblast growth factor (bFGF) expression and mitogenic effects were assessed in bovine aortic ECs incubated with oxidizedLDL (ox-LDL). Compared with native LDL and Lipoprotein-free controls, ox-LDL reduced bFGF mRNA levels in a time- and concentration-dependent manner, 100 mu g/mL producing a maximum reduction of 40% to 50% within 24 to 48 hours. There were commensurate reductions in intracellular and extracellular bFGF concentrations, DNA and total RNA syntheses, and cell replication. FGF receptor 1 and beta-actin mRNA levels were unchanged. Ox-LDL accelerated bFGF mRNA degradation in actinomycin D-treated cells. However, inhibition of bFGF expression by ox-LDL was attenuated by cyclohexamide, indicating a requirement for continuous new protein synthesis for posttranscriptional destabilization, Reduced syntheses of DNA and total RNA were completely restoredby bFGF but not by vascular endothelial growth factor. Inhibition of totalRNA synthesis achieved by exposing cells to a bFGF-neutralizing antibody was similar in magnitude: to that induced by ox-LDL. Conclusions-Cytotoxic effects of ox-LDL on ECs are attributable in part tosuppression of bFGF expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 00:24:02