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Titolo:
Nitrotyrosine generation via inducible nitric oxide synthase in vascular wall in focal ischemia-reperfusion
Autore:
Hirabayashi, H; Takizawa, S; Fukuyama, N; Nakazawa, H; Shinohara, Y;
Indirizzi:
Tokai Univ, Sch Med, Dept Neurol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 , Dept Neurol, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Physiol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 Dept Physiol, Kanagawa 2591193, Japan
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 852, anno: 2000,
pagine: 319 - 325
SICI:
0006-8993(20000110)852:2<319:NGVINO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL-ISCHEMIA; RADICAL PRODUCTION; MICE LACKING; RAT; PEROXYNITRITE; INJURY; BRAIN; GENE; 3-NITROTYROSINE; NEUTROPHILS;
Keywords:
brain ischemia; endothelium; nitric oxide; peroxynitrite; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Shinohara, Y Tokai Univ, Sch Med, Dept Neurol, Boseidai, Kanagawa 2591193,Japan Tokai Univ Boseidai Kanagawa Japan 2591193 wa 2591193, Japan
Citazione:
H. Hirabayashi et al., "Nitrotyrosine generation via inducible nitric oxide synthase in vascular wall in focal ischemia-reperfusion", BRAIN RES, 852(2), 2000, pp. 319-325

Abstract

Nitrotyrosine produced by NO-mediated reaction is a possible marker for cytotoxicity in brain ischemia. In this study, we aimed to determine whether iNOS is responsible for the nitrotyrosine formation and which type of cell is predominantly nitrated. Fifty-eight wild-type and 28 iNOS knockout male mice were used. Under halothane anesthesia the left middle cerebral artery was occluded for 2 h and reperfused for 0.5 or 15 h. The ratio of nitrotyrosine to total tyrosine (%NO2-Tyr) was measured by means of a hydrolysis/HPLC. After 0.5-h reperfusion, %NO2-Tyr in the ischemic cortex of wild-type and knockout mice amounted to 0.037 +/- 0.040% (n = 8) and 0.064 +/- 0.035% (n = 6), respectively, being significantly higher than that in the sham operation group (n = 7) (P < 0.05). After 15-h reperfusion, nitrotyrosine was detected only in wild-type mice (0.039 +/- 0.025%, n = 7), not in knockout or sham-operated mice (P < 0.05), Immunohistochemical reaction for nitrotyrosine was seen predominantly in the vascular wall in the peri-infarct regionof the cerebral cortex in wild-type mice after 15-h reperfusion, but not in corresponding knockout mice. Our data suggest that iNOS is responsible for nitrotyrosine formation in the later phase of reperfusion, and that vascular endothelium is the major site of this reaction, at least in the case of15-h reperfusion. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:07:58