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Titolo:
Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells
Autore:
Lin, WW; Friedman, MA; Wang, XF; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol, Lab Neurotoxicol, Durham, NC 27710 USADuke Univ Durham NC USA 27710 col, Lab Neurotoxicol, Durham, NC 27710 USA Univ Med & Dent New Jersey, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 rsey, Newark, NJ 07103 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 852, anno: 2000,
pagine: 297 - 304
SICI:
0006-8993(20000110)852:2<297:ANEIRP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PC12 CELLS; GENE-EXPRESSION; PROTEINS; NEUROTOXICITY; NEURONS; MOTOR; MODEL; BRAIN;
Keywords:
neurofilament; acrylamide; neurotoxicity; gene expression; nerve growth factor; PC12 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol, Lab Neurotoxicol, Box 3813, Durham, NC27710 USA Duke Univ Box 3813 Durham NC USA 27710 Durham, NC27710 USA
Citazione:
W.W. Lin et al., "Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells", BRAIN RES, 852(2), 2000, pp. 297-304

Abstract

Using the rat pheochromocytoma cell line (PC12), we present molecular evidence that the neurotoxicant acrylamide directly induces neurofilament gene expression, and the signaling pathways are initially distinctive from, but eventually merged into, that for nerve growth factor (NGF)-induced neurofilament expression. In PC12 cells, acrylamide increased neurofilament proteinlevels and synthesis. Acrylamide had no effect on the stability of neurofilament mRNAs suggesting that it directly increased neurofilament mRNA synthesis. K252a, a selective inhibitor for NGF receptor gp140trk, had no effecton acrylamide induction, but completely inhibited NGF-induced neurofilament protein synthesis. Therefore, the initial step for acrylamide signaling was distinctive from NGF. Dexamethasone reversed the effects of both NGF andacrylamide on neurofilament protein levels and synthesis indicated that there is a dexamethasone-sensitive signaling step upon which NGF and acrylamide merge, suggesting involvement of transcription-activating proteins like AP-1. These results, taken together with previous studies of transgenic mice that overexpress neurofilament genes, may partially explain the mechanisms of neurofilament accumulation in distal parts of large axons, a pathognomonic feature of acrylamide neurotoxicity in animals. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 10/07/20 alle ore 00:20:56