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Titolo:
Monte Carlo simulation of 3-D buffered Ca2+ neuroendocrine cells
Autore:
Gil, A; Segura, J; Pertusa, JAG; Soria, B;
Indirizzi:
Univ Miguel Hernandez, Dept Ciencias, Expt & Tecnol Edificio La Galia, Inst Bioingn, Alicante 03202, Spain Univ Miguel Hernandez Alicante Spain 03202 ioingn, Alicante 03202, Spain
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 1, volume: 78, anno: 2000,
parte:, 1
pagine: 13 - 33
SICI:
0006-3495(200001)78:1<13:MCSO3B>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL CHROMAFFIN CELLS; PANCREATIC B-CELLS; TRANSMITTER RELEASE; ANOMALOUS DIFFUSION; SECRETORY GRANULES; CALCIUM; BINDING; MICRODOMAINS; EXOCYTOSIS; CHANNELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Segura, J Univ Miguel Hernandez, Dept Ciencias, Expt & Tecnol Edificio La Galia, Inst Bioingn, Alicante 03202, Spain Univ Miguel Hernandez AlicanteSpain 03202 icante 03202, Spain
Citazione:
A. Gil et al., "Monte Carlo simulation of 3-D buffered Ca2+ neuroendocrine cells", BIOPHYS J, 78(1), 2000, pp. 13-33

Abstract

Buffered Ca2+ diffusion in the cytosol of neuroendocrine cells is a plausible explanation for the slowness and latency in the secretion of hormones. We have developed a Monte Carlo simulation to treat the problem of 3-D diffusion and kinetic reactions of ions and buffers. The 3-D diffusion is modeled as a random walk process that follows the path of each ion and buffer molecule, combined locally with a stochastic treatment of the first-order kinetic reactions involved. Such modeling is able to predict [Ca2+] and bufferconcentration time courses regardless of how low the calcium influx is, and it is therefore a convenient method for dealing with physiological calcium currents and concentrations. We study the effects of the diffusional and kinetic parameters of the model on the concentration time courses as well as on the local equilibrium of buffers with calcium. An in-mobile and fast endogenous buffer as described by Klingauf and Neher (1997, Biophys. J. 72:674-690) was able to reach local equilibrium with calcium; however, the exogenous buffers considered are displaced drastically from equilibrium at the start of the calcium pulse, particularly below the pores. The versatility of the method also allows the effect of different arrangements of calcium channels on submembrane gradients to be studied, including random distribution of calcium channels and channel clusters. The simulation shows how the particular distribution of channels or clusters can be of relevance for secretion in the case where the distribution of release granules is correlated with the channels or clusters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:04:22