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Titolo:
Different recognition by peroxisome proliferator structures in rat peroxisomal induction: Application of sandwich ELISA using monoclonal antibody against rat peroxisomes
Autore:
Kawano, H; Nishi, F; Kamitani, Y; Ochi, H; Miyake, M; Mayumi, T; Hama, T;
Indirizzi:
Kobe Gakuin Univ, Fac Pharmaceut Sci, Nishi Ku, Kobe, Hyogo 6512180, JapanKobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Osaka Univ, Fac Pharm, Suita, Osaka 565, Japan Osaka Univ Suita Osaka Japan 565 Univ, Fac Pharm, Suita, Osaka 565, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 1, volume: 23, anno: 2000,
pagine: 12 - 16
SICI:
0918-6158(200001)23:1<12:DRBPPS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED RECEPTOR-ALPHA; RETINOID-X-RECEPTOR; ACYL-COA OXIDASE; FATTY-ACIDS; HYPOLIPIDEMIC DRUGS; GENE-EXPRESSION; BETA-OXIDATION; LIVER; EICOSANOIDS; SYSTEM;
Keywords:
peroxisomal beta-oxidation; peroxisomal bifunctional enzyme; sandwich ELISA; peroxisome proliferator; co-treatment; rat hepatocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Kawano, H Kobe Gakuin Univ, Fac Pharmaceut Sci, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 Hyogo 6512180, Japan
Citazione:
H. Kawano et al., "Different recognition by peroxisome proliferator structures in rat peroxisomal induction: Application of sandwich ELISA using monoclonal antibody against rat peroxisomes", BIOL PHAR B, 23(1), 2000, pp. 12-16

Abstract

A novel assay for a peroxisomal beta-oxidation enzyme by sandwich ELISA using a monoclonal antibody (RPX-5) against purified rat liver peroxisomes was developed. Immunoblot analysis revealed that RPX-5 recognized a 78 Kd protein, which is a peroxisomal bifunctional enzyme (PBE) in the beta-oxidation pathway, Immunoprecipitation by RPX-5 and the resulting reduction of PBE activity were dependent on RPX-5 concentrations. Sandwich ELISA using RPX-5could be used to assay PBE in the range of 30 to 2000 ng protein/ml. in rat hepatocyte cultures, the PBE amount by this assay correlated well with PBE activity with correlation coefficients of 0.965, Studying the mechanisms of peroxisomal induction, patterns of peroxisomal induction were examined by co-treatment of rat hepatocytes with various peroxisome proliferators (PxPs), Treatment with clofibrate and bezafibrate resulted in neither an additive nor synergistic effect on PBE level. On the other hand, co-treatment with either bezafibrate-Wy-14,643 or clofibrate-MEHP(mono(2-ethylhexyl)phthalate both resulted in an additive effect. From these results, it is suggested that PxPs of the fibrate group may exert their functions via a common process, and non-fibrate PxPs via a different process in hepatocytes, The cognition site for peroxisome proliferators, therefore, might not involve a single site for inducing peroxisomal enzymes,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:20:01