Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cloning and functional characterization of a Na+-independent, broad-specific neutral amino acid transporter from mammalian intestine
Autore:
Rajan, DP; Kekuda, R; Huang, W; Devoe, LD; Leibach, FH; Prasad, PD; Ganapathy, V;
Indirizzi:
Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 m & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia, Dept Obstet & Gynecol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 et & Gynecol, Augusta, GA 30912 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 1, volume: 1463, anno: 2000,
pagine: 6 - 14
SICI:
0005-2736(20000115)1463:1<6:CAFCOA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHORIOCARCINOMA CELL-LINE; HEAVY-CHAIN; SYSTEM-L; MODULATION; EXPRESSION; ANTIGEN; PROTEIN; Y(+)L;
Keywords:
amino acid transport; system L; LAT2; 4F2 heavy chain; primary structure; functional expression; rabbit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ganapathy, V Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 Augusta, GA 30912 USA
Citazione:
D.P. Rajan et al., "Cloning and functional characterization of a Na+-independent, broad-specific neutral amino acid transporter from mammalian intestine", BBA-BIOMEMB, 1463(1), 2000, pp. 6-14

Abstract

We have isolated a cDNA from a rabbit intestinal cDNA library which, when co-expressed with the heavy chain of the human 4F2 antigen (4F2hc) in mammalian cells, induces system L-like amino acid transport activity. This protein, called LAT2, consists of 535 amino acids and is distinct from LAT1 which also interacts with 4F2hc to induce system L-like amino acid transport activity. LAT2 does not interact with rBAT, a protein with a significant structural similarity to 4F2hc. The 4F2hc/LAT2-mediated transport process differs from the 4F2hc/LAT1-mediated transport in substrate specificity, substrate affinity, tissue distribution, interaction with D-amino acids, and pH-dependence. The 4F2hc/LAT2-associated transport process has a broad specificity towards neutral amino acids with K-t values in the range of 100-1000 mu M, does not interact with D-amino acids to any significant extent, and is stimulated by acidic pH. In contrast, the 4F2hc/LAT1-associated transport process has a narrower specificity towards neutral amino acids, but with comparatively higher affinity (K-t values in the range of 10-20 mu M), interacts with some D-amino acids with high affinity, and is not influenced by pH. LAT2 is expressed primarily in the small intestine and kidney, whereas LAT1exhibits a much broader tissue distribution. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 08:41:57