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Titolo:
Tumor necrosis factor alpha promotes the expression of stem cell factor insynovial fibroblasts and their capacity to induce mast cell chemotaxis
Autore:
Kiener, HP; Hofbauer, R; Tohidast-Akrad, M; Walchshofer, S; Redlich, K; Bitzan, P; Kapiotis, S; Steiner, G; Smolen, JS; Valent, P;
Indirizzi:
Univ Vienna, Vienna Gen Hosp, Dept Internal Med 3, Div Rheumatol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Div Rheumatol, A-1090 Vienna, Austria
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 1, volume: 43, anno: 2000,
pagine: 164 - 174
SICI:
0004-3591(200001)43:1<164:TNFAPT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; RHEUMATOID-ARTHRITIS; RECOMBINANT HUMAN; MEDIATOR RELEASE; GROWTH-FACTOR; BONE-MARROW; ACTIVATION; RECEPTOR; TRYPTASE; IGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Kiener, HP Univ Vienna, Vienna Gen Hosp, Dept Internal Med 3, Div Rheumatol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 stria
Citazione:
H.P. Kiener et al., "Tumor necrosis factor alpha promotes the expression of stem cell factor insynovial fibroblasts and their capacity to induce mast cell chemotaxis", ARTH RHEUM, 43(1), 2000, pp. 164-174

Abstract

Objective. To investigate the expression of the stroma cell product stem cell factor (SCF) in synovial fibroblasts (SFB) in patients with rheumatoid arthritis (RA) and osteoarthritis (OA), and to analyze the capacity of SFB to induce mast cell (MC) chemotaxis,Methods. Synovial tissue was obtained from 29 patients with RA and 25 patients with OA, Tissue was dispersed by enzymatic digestion using collagenase, SFB were grown in serial passage and exposed to tumor necrosis factor `alpha (TNF alpha) or control medium, Expression of SCF in cultured SFB was analyzed by reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and immunostaining. The ability of SFB (supernatants) to induce MC migration was analyzed using a double-chamber chemotaxis assay and the human mast cell line HMC-1. In situ expression of SCF in synovial tissue from patients with RA (n = 6) and OA (n = 6) was examined by double immunohistochemistry using antibodies against SCF and the fibroblast-specific antibody ASO2,Results. In both RA and OA, cultured SFB were found to express SCF messenger RNA, as assessed by RT-PCR, In addition, the SCF protein was detectable in cell lysates and supernatants of SFB by ELISA, Incubation of SFB with TNF alpha resulted in an increased expression and release of SCF, Recombinanthuman SCF (rHuSCF) and SFB supernatants induced significant migration of HMC-1 cells above control levels. In addition, exposure of SFB to TNF alpha led to an increased migration of HMC-1, and a blocking anti-SCF antibody inhibited the rHuSCF- and SFB-induced migration of HMC-1. In situ double immunostaining revealed expression of SCF in ASO2-positive SFB in the synovium of patients with RA. Conclusion. Our results show that SFB (in RA and OA) express SCF and induce MC chemotaxis, Furthermore, TNF alpha was found to augment SCF expressionin SFB, It is hypothesized that these cellular interactions play an important role in MC accumulation and related events in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 20:53:26