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Titolo:
DETECTION OF MALIGNANT-CELLS IN CEREBROSPINAL-FLUID USING FLUORESCENCE IN-SITU HYBRIDIZATION
Autore:
VANOOSTENBRUGGE RJ; HOPMAN AHN; LENDERS MH; VANHEERDE P; ARENDS JW; RAMAEKERS FCS; TWIJNSTRA A;
Indirizzi:
UNIV HOSP MAASTRICHT,DEPT NEUROL,POB 5800 NL-6202 AZ MAASTRICHT NETHERLANDS UNIV HOSP MAASTRICHT,DEPT PATHOL NL-6202 AZ MAASTRICHT NETHERLANDS MAASTRICHT UNIV,DEPT MOL GENET & CELL BIOL MAASTRICHT NETHERLANDS NETHERLANDS CANC INST,DEPT PATHOL NL-1066 CX AMSTERDAM NETHERLANDS
Titolo Testata:
Journal of neuropathology and experimental neurology
fascicolo: 6, volume: 56, anno: 1997,
pagine: 743 - 748
SICI:
0022-3069(1997)56:6<743:DOMICU>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU; MENINGEAL CARCINOMATOSIS; INTERPHASE CYTOGENETICS; INSITU HYBRIDIZATION; LEPTOMENINGEAL METASTASES; FLOW-CYTOMETRY; CANCER; DIAGNOSIS; CYTOLOGY; TUMORS;
Keywords:
CEREBROSPINAL FLUID; DIAGNOSTIC CYTOLOGY; FLUORESCENCE IN SITU HYBRIDIZATION; LEPTOMENINGEAL METASTASIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Vanoostenbrugge et al., "DETECTION OF MALIGNANT-CELLS IN CEREBROSPINAL-FLUID USING FLUORESCENCE IN-SITU HYBRIDIZATION", Journal of neuropathology and experimental neurology, 56(6), 1997, pp. 743-748

Abstract

Cytologic examination of cerebrospinal fluid (CSF) is the diagnostic gold standard for leptomeningeal metastasis (LMM). However, this technique is only moderately sensitive when routine staining procedures areapplied. The use of fluorescence in situ hybridization (FISH) to identify malignant cells may have an additional value in diagnosing LMM, since numerical chromosomal aberrations (NCA) can be detected at the single cell level. We tested the feasibility of FISH to detect tumor cells in CSF by analyzing 22 samples with proven LMM with a probe for chromosome 1 (1q12) to detect NCA in the cells. A control group consistedof samples from 10 patients with inflammatory neurologic disease. In 7 LMM samples no cells or only lysed cells were found, due to lime delay before fixation. Of the other 15 LMM samples analyzed, 13 showed NCA (87%), while no NCA were found in the control group. Our results indicate that FISH may be a useful additional diagnostic tool to the cytodiagnosis of CSF in cases of LMM. We expect that FISH can become an additional marker for malignancy at the single cell level in patients with LMM, which may also be of use to determine the effect of therapy for LMM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:47:26