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Titolo:
Automated high-throughput liquid-liquid extraction for initial purification of combinatorial libraries
Autore:
Peng, SX; Henson, C; Strojnowski, MJ; Golebiowski, A; Klopfenstein, SR;
Indirizzi:
Procter & Gamble Co, Hlth Care Res Ctr, Mason, OH 45040 USA Procter & Gamble Co Mason OH USA 45040 Care Res Ctr, Mason, OH 45040 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 2, volume: 72, anno: 2000,
pagine: 261 - 266
SICI:
0003-2700(20000115)72:2<261:AHLEFI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MASS-SPECTROMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Peng, SX Procter & Gamble Co, Hlth Care Res Ctr, 8700 Mason Montgomery Rd,Mason, OH 45040 USA Procter & Gamble Co 8700 Mason Montgomery Rd Mason OH USA 45040 A
Citazione:
S.X. Peng et al., "Automated high-throughput liquid-liquid extraction for initial purification of combinatorial libraries", ANALYT CHEM, 72(2), 2000, pp. 261-266

Abstract

An automated high-throughput Liquid-liquid extraction (LLE) methodology has been developed and utilized for the initial purification of the combinatorial library samples containing unreacted amines and other water-soluble byproducts or impurities. Various extraction solvents were evaluated along with different extraction devices. The LLE method was automated using 96-well-format plates and a robotic Liquid-handling workstation. In the optimized LLE method, crude combinatorial library samples were dissolved in a water-immiscible organic solvent, butyl acetate, and added to each well in a 96-well-format plate packed with an inert support material coated with hydrochloric acid. Separation occurs based on the partitioning of the compounds between two liquid phases. Product recovery, purity, and amine removal efficiency were determined by HPLC with and without precolumn derivatization, The automated method was successfully applied to the cleanup of some representative combinatorial library samples with greater than 98% amine removal and an average product purity of 90%, The application of the automated high-throughput LLE method should greatly reduce the labor, time, and cost associated with the purification of combinatorial libraries.

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Documento generato il 04/12/20 alle ore 03:48:54