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Titolo:
Protein tyrosine kinase regulates the number of renal secretory K channels
Autore:
Wang, WH; Lerea, KM; Chan, M; Giebisch, G;
Indirizzi:
New York Med Coll, Dept Pharmacol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 Pharmacol, Valhalla, NY 10595 USA New York Med Coll, Dept Anat & Cell Biol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 Cell Biol, Valhalla, NY 10595 USA Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 r & Mol Physiol, New Haven, CT 06510 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 1, volume: 278, anno: 2000,
pagine: F165 - F171
SICI:
0363-6127(200001)278:1<F165:PTKRTN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING TUBULE; POTASSIUM CHANNEL; APICAL MEMBRANE; RAT-KIDNEY; RECEPTOR; TRANSPORT; SRC; ALDOSTERONE; ACTIVATION; ADAPTATION;
Keywords:
potassium depletion; high-potassium intake; potassium secretion; cortical collecting duct;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, WH New York Med Coll, Dept Pharmacol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 , Valhalla, NY 10595 USA
Citazione:
W.H. Wang et al., "Protein tyrosine kinase regulates the number of renal secretory K channels", AM J P-REN, 278(1), 2000, pp. F165-F171

Abstract

The apical small conductance (SK) channel plays a key role in K secretion in the cortical collecting duct (CCD). A high-K intake stimulates renal K secretion and involves a significant increase in the number of SK channels in the apical membrane of the CCD. We used the patch-clamp technique to examine the role of protein tyrosine kinase (PTK) in regulating the activity ofSK channels in the CCD. The application of 100 mu M genistein stimulated SK channels in 11 of 12 patches in CCDs from rats on a K-deficient diet, andthe mean increase in NP0, a product of channel number (N) end open probability (P-0), was 2.5. In contrast, inhibition of PTK had no effect in tubules from animals on a high-K diet in all 10 experiments. Western blot analysis further shows that the level of cSrc, a nonreceptor type of PTK is 261% higher in the kidneys from rats on a K-deficient diet than those on a high-Kdiet. However, the effect of cSrc was not the result of direct inhibition of channel itself, because addition elf exogenous cSrc had no effect on SK channels in inside-out patches. In cell-attached patches, application of herbimycin A increased channel activity in 14 of 16 patches, and the mean increase in NP0 was 2.4 in tubules from rats on a K-deficient diet. In contrast, herbimycin A had no effect on channel activity in any of 15 tubules fromrats on a high-K diet. Furthermore, herbimycin A pretreatment increased NP0 per patch from the control value (0.4) to 2.25 in CCDs from rats on a K-deficient diet, whereas herbimycin failed to increase channel activity (NP0:control, 3.10; herbimycin A, 3.25) in the CCDs from animals on a high-K diet. We conclude that PTK is involved in regulating the number of apical SK channels in the kidney.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:30:52