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Titolo:
Developmental changes in endothelial nitric oxide synthase expression and activity in ovine fetal lung
Autore:
Parker, TA; Le Cras, TD; Kinsella, JP; Abman, SH;
Indirizzi:
Univ Colorado, Sch Med, Pediat Heart Lung Ctr, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 at Heart Lung Ctr, Denver, CO 80262 USA Univ Colorado, Sch Med, Div Neonatol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Med, Div Neonatol, Denver, CO 80262 USA Univ Colorado, Sch Med, Div Pulm & Crit Care Med, Dept Pediat, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Med, Dept Pediat, Denver, CO 80262 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 278, anno: 2000,
pagine: L202 - L208
SICI:
1040-0605(200001)278:1<L202:DCIENO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; RAT LUNG; PULMONARY CIRCULATION; MATURATIONAL CHANGES; POSTNATAL-GROWTH; GENE-EXPRESSION; RELAXING FACTOR; OXYGEN; FETUS; ANGIOGENESIS;
Keywords:
lung development; pulmonary vasculature; angiogenesis; pulmonary vasoreactivity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Parker, TA Childrens Hosp, Div Neonatol, Box 070,1056 E 19th Ave, Denver, CO 80218 USA Childrens Hosp Box 070,1056 E 19th Ave Denver CO USA 80218 USA
Citazione:
T.A. Parker et al., "Developmental changes in endothelial nitric oxide synthase expression and activity in ovine fetal lung", AM J P-LUNG, 278(1), 2000, pp. L202-L208

Abstract

Endothelial nitric oxide (NO) synthase (eNOS) produces NO, which contributes to vascular reactivity in the fetal lung. Pulmonary vasoreactivity develops during late gestation in the ovine fetal lung, during the period of rapid capillary and alveolar growth. Although eNOS expression peaks near birthin the fetal rat, lung capillary and distal air space development occur much later than in the fetal lamb. To determine whether lung eNOS expression in the lamb differs from the timing and pattern reported in the rat, we measured eNOS mRNA and protein by Northern and Western blot analyses and NOS activity by the arginine-to-citrulline conversion assay in lung tissue from fetal, newborn, and maternal sheep. Cellular localization of eNOS expression was determined by immunohistochemistry. eNOS mRNA, protein, and activity were detected in samples from all ages, and eNOS was expressed predominantly in the vascular endothelium. Lung eNOS mRNA expression increases from lowlevels at 70 days gestation to peak at 113 days and remains high for the rest of fetal life. Newborn eNOS mRNA expression does not change from fetal levels but is lower in the adult ewe. Lung eNOS protein expression in the fetus rises and peaks at 118 days gestation but decreases before birth. eNOSprotein expression rises in the newborn period but is lower in the adult. Lung NOS activity also peaks at 118 days gestation in the fetus before falling in late gestation and remaining low in the newborn and adult. We conclude that the pattern of lung eNOS expression in the sheep differs from that in the rat and may reflect species-related differences in lung development. We speculate that the rise in fetal lung eNOS may contribute to the markedlung growth and angiogenesis that occurs during the same period of time.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:27:12