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Titolo:
Use of atp6 in fungal phylogenetics: An example from the Boletales
Autore:
Kretzer, AM; Bruns, TD;
Indirizzi:
Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 al Biol, Berkeley, CA 94720 USA
Titolo Testata:
MOLECULAR PHYLOGENETICS AND EVOLUTION
fascicolo: 3, volume: 13, anno: 1999,
pagine: 483 - 492
SICI:
1055-7903(199912)13:3<483:UOAIFP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL DNA-SEQUENCES; GROUP-I INTRON; GENE; EVOLUTION; SUILLUS; NUCLEAR; MUSHROOMS; IDENTIFICATION; GASTROSUILLUS; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Kretzer, AM Oregon State Univ, Dept Bot & Plant Pathol, 2082 Cordley Hall,Corvallis, OR 97331 USA Oregon State Univ 2082 Cordley Hall Corvallis OR USA 97331 USA
Citazione:
A.M. Kretzer e T.D. Bruns, "Use of atp6 in fungal phylogenetics: An example from the Boletales", MOL PHYL EV, 13(3), 1999, pp. 483-492

Abstract

Complete nucleotide sequences have been determined for atp6 from Suillus luteus and cox3 from Suillus sinuspaulianus (Boletales, Hymenomycetes, Basidiomycota), which code for ATPase subunit 6 and cytochrome oxidase subunit 3, respectively. These sequences were used to design PCR primers for the amplification of partial atp6 and cox3 sequences from other members of the Boletales and outgroup taxa. In atp6 and coxs from Russula rosacea, one of theoutgroup taxa, we observed a number of in-frame TGA(trp) codons, which imply a Neurospora crassa-type mitochondrial code in R. rosacea and possibly in basidiomycetes in general. Interestingly, however, most basidiomycetes other than R. rosacea appear to strongly prefer the TGG(trp) codon, which is unusual, given the strong A + T bias in fungal mitochondrial genomes. Pairwise comparisons were performed between atp6 sequences from increasingly divergent fungal lineages, and results show that all three codon positions become saturated in substitutions after an estimated divergence time of approx300 Ma. This means that atp6 is likely to provide phylogenetic resolution within fungal classes but not at higher taxonomic levels. Also, because of the strong A + T bias in fungal mitochondrial genomes, APT transversions were found to be more common than any other type of substitution, resulting in transversions being about two to three times more common in most pairwisesequence comparisons. Finally, atp6 sequences were used to infer phylogenetic relationships between 27 taxa from the Boletales and 4 outgroup taxa. Analyses were performed (i) on nucleotide sequence data using parsimony (successive approximation) as well as maximum likelihood methods and (ii) on deduced amino acid sequences using distance methods based on empirical substitution probabilities. Results from the various analyses are largely concordant with each other as well as with prior analyses of partial mitochondriallarge-subunit rDNA (mtLSU rDNA). Analysis of the combined atp6 and mtLSU rDNA sequences results in increased bootstrap support for several key branches. Relationships that have been resolved for the first time in the currentanalysis are discussed. (C) 1999 Academic Press.

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Documento generato il 02/04/20 alle ore 19:18:37